Excellgen/Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)/EG-1001/10 mg,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)/EG-1001/10 mg

产品编号：EG-1001-10mg

市场价：¥70000.00

场地：美国(厂家直采)

产品分类：细胞相关>细胞培养>添加物>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$3500.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Cat#	EG-1001(oldcatalog#:RP-7)
Discount	$100DiscountCodeforYourFirstOrderof1mgCreRecombinase:CreDiscount Discountforswitchingfromcompetitors'product:$150.EmailtoTechSupportts@Excellgen.comfordiscountcode.
Description	Crerecombinase,oftenabbreviatedtoCre,isaTypeItopoisomerasefromP1bacteriophagethatcatalyzessite-specificrecombinationofDNAbetweenloxPsites.Thisenzymedoesnotrequireanyenergycofactors,andCre-mediatedrecombinationquicklyreachesequilibriumbetweensubstrateandreactionproducts.TheloxPrecognitionelementisa34basepair(bp)sequencecomposedoftwo13bpinvertedrepeatsflankingan8bpspacerregionwhichconfersdirectionality.RecombinationproductsaredependentonthelocationandrelativeorientationoftheloxPsites.TwoDNAspeciescontainingsingleloxPsiteswillbefusedwhilstDNAbetweenloxPsitesinthesameorientationwillbeexcisedincircularformandDNAbetweenopposingloxPsiteswillbeinvertedwithrespecttotherestoftheDNA. Crerecombinaseisusedasatooltomodifygenesandchromosomes.InthisapproachtheCrerecombinaseisusedtodeleteasegmentofDNAflankedbyLoxPsites(aka'floxed')inanexperimentalanimal.Ithasbeenusedtogenerateanimalswithmutationslimitedtocertaincelltypes(tissue-specificknockout)oranimalswithmutationsthatcanbeactivatedbydrugadmiNISTration(inducIBLeknockout)inanumberoftransgenicspecies.TheavailABIlityoftransgeniclineswithtissuespecificorinducibleCreexpressionpermitsresearcherstoinactivateoractivateageneofinterestsimplybybreedingafloxedanimaltopre-existingCre-transgenics.OneexampleofaninducibleCrerecombinasesystemistheCre-ER(ER=EstrogenReceptor)systeminwhichintraperitonealinjectionoftamoxifenwillcausedose-dependentexcisionofthefloxedsite(i.e.willinactivatethegeneofchoice). RecombinantCrerecombinase(TAT-Cre)waspurifiedfromanE.colistraincarryinganengineeredplasmidencodingenhancedformofCreRecombinasefrombacteriophageP1.ThisCrerecombinasehasanN-terminal6XHistag,aTatpeptide(GRKKRRQRRRPPAGTSVSL)andanNLSsequence(PKKKRKV).HTNCisthemosteffectiveproteinintransduction(invivo)andsubsequentrecombinationcomparedtootherformsofCrerecombinases,e.g.,HNC,TCH6,HC,HNCM,CH.Incubationoffibroblastreportercellswith1μMHTNCfor1to2hourscanresultintranductionof60~90%ofthecells.Additionof100μMchoroquinetoculturemediummay furtherenhancetransductionandrecombination. Usethispagetocalculateconcentrationrequiredforcultured cells: http://www.excellgen.com/pub/mw_2_moles.html
Applications	InvitroLoxPrecombinationforsubcloningorvector/cloneengineering TransductionintoculturedcellsincludingstemcellsexVivo
Properties	Concentration:2,5,or10mg/ml. MolecularWeight:43kDa QC:Tat-Creinduced80~100%recombinationefficienciesinHEK293T-Crereportercells.HEK293T-cellsweretransfectedwithreporterplasmidDNA:LoxP-RFP-Stop-LoxP-GFP,thenadd1μMpurifiedTat-Creprotein. Fig1:0:beforeaddingTat-Cre;1to5:1to5daysaftertransductionwithTat-Cre. EndotoxinLevels:<0.1EU/μg Purity:>98%bySDS-PAGEandHPLCanalysis.
UnitDefintion	Astandardof100UnitsisdefinedastheamountofTAT-CRE(μg) in1.0mLoftissueculturemediumthatisrequiredtoinduce50% GFPexpressioninaHEK293Treportercelllineassay.
Stability	2yearsat-80^oC.6monthsat-20^oC.Upto3weeksat4^oC
Citations	1.Integrin-LinkedKinaseRegulatesProcessExtensioninOligodendrocytesviaControlofActinCytoskeletalDynamics,TheJournalofNeuroscience,5June2013,33(23):9781-9793;doi:10.1523/JNEUROSCI.5582-12.2013 2.CuttingEdge:Krüppel-likeFactor2IsRequiredforPhenotypicMaintenancebutNotDevelopmentofB1BCells.TheJournalofImmunology. August31,20121201439.doi:10.4049/jimmunol.1201439.http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3448866/ 3.KINASE-INDEPENDENTFEEDBACKOFTHETAK1/TAB1COMPLEXONBCL10TURNOVERANDNF-κBACTIVATION.Mol.Cell.Biol.2013.doi:10.1128/MCB.06407-11 4.Defectiveglucosemetabolisminpolycystickidneydiseaseidentifiesanewtherapeuticstrategy.NatureMedicine19,488–493(2013)doi:10.1038/nm.3092 5.TCell−DerivedIL-17MediatesEpithelialChangesintheAirwayandDrivesPulmonaryNeutrophilia.JImmunolpublishedonline21August2013http://www.jimmunol.org/content/early/2013/08/21/jimmunol.1301360 6.RapidconversionofEUCOMM/KOMP-CSDallelesinmouseembryosusingacell-permeableCrerecombinase.TransgenicResearch.February2014,Volume23,Issue1,pp177-185.doi:10.1007/s11248-013-9764-xNCBI:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3890051/ 7.Antibody-membraneswitch(AMS)technologyforfacilecelllinedevelopment. ProteinEngineering,DesignandSelection(2014)27(10):309-315.doi:10.1093/protein/gzu039 8.TheroleofantisenselongnoncodingRNAinsmallRNA-triggeredgeneactivation.RNA.October24,2014.doi:10.1261/rna.043968.113 9.ASingleOncogenicEnhancerRearrangementCausesConcomitantEVI1andGATA2DeregulationinLeukemia.Cell.Volume157,Issue2,p369–381,10April2014.DOI:10.1016/j.cell.2014.02.019
Storage	-20ºC~-80ºC
Shipping	dryice

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
Excellgen	GeneExpresso™Max Transfection Reagent	2	EG-1089	1ml
Excellgen	Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)	4	EG-1069	1 mg
Excellgen	Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg
Excellgen	serine/threonine kinase 33, STK33	7	EG-261	100 µg
Excellgen	Alpha-tubulin Rabbit Polyclonal Antibody	9	EG-1000	100 µg
Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
Excellgen	Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts	13	EG-266	100 µg
Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">