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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/p53, Recombinant wild-type human p53 protein/RP-100/5,000 U
产品编号:RP-100
市  场 价:¥6000.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$300.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/p53, Recombinant wild-type human p53 protein/RP-100/5,000 U
商品介绍
ProductNameRecombinantwild-typehumanp53protein
Size5,000U
Description

Thisgeneencodestumorproteinp53,whichrespondstodiversecellularstressestoregulatetargetgenesthatinducecellcyclearrest,apoptosis,senescence,DNArepair,orchangesinmetabolism.p53proteinisexpressedatlowlevelinnormalcellsandatahighlevelinavarietyoftransformedcelllines,whereit'sbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingtranscriptionactivation,DNA-binding,andoligomerizationdomains.Itispostulatedtobindtoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.Alterationsofthisgeneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.Multiplep53variantsduetoalternativepromotersandmultiplealternativesplicinghavebeenfound.Thesevariantsencodedistinctisoforms,whichcanregulatep53transcriptionalactivity.

Humanwild-typep53proteiniscomposedof393aminoacidresidueswithseveraldistinctregions.TheN-terminalactivationdomainallowsp53proteintorecruitthebasaltranscriptionmachineryandactivatetheexpressionoftargetgenes,whereasthecoredomainbindstotargetDNAinasequence-specificmannerandthemajorityofmutationsfoundinhumantumorsoccurintheregionofthegeneencodingthisdomain(1-3).TheC-terminaldomainiscomposedofpredominantlybasicresiduesandmodificationoftheC-terminalbasicdomain,includingacetylation,glycosylationandphosphorylation,isanessentialmechanismforregulatingp53function(4-6).

Applications
  • gelmobilityshiftassayorforaDNaseIfootprintingassayinthepresenceofdoublestrandedDNAcontainingaconsensusp53-bindingsequence[5’-PuPuPuC(A/T)(T/A)GPyPyPy-3’]
  • invitrotranscriptionassay
  • protein-proteininteractionassay
  • forcellgrowthassay
Sourcebaculovirus/sf9cells
FusionTag6XHis
PurificationMethodFPLC
UnitDefinition(Activity)

1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay.

Purity

~95%asdeterminedbySDS-PAGE

GeneID7157
Accession#NM_000546
GeneNametumorproteinp53
GeneSymbolTP53
GeneSynonymsP53;BCC7;LFS1;TRP53
MW45.2kDa
ProteinSequence

EAPRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKS
VTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHER
CSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSS
CMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPP
GSTKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGG
SRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD

StorageBuffer20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage-20to-80°C.
Shipping4°Cordryice
References1.Finlay,C.etal.,(1989)Cell57,1083-1093
2.Michalovitz,D.etal.,(1990)Cell62,671-680
3.Baker,S.etal.,(1990)Science249,912-915
4.Fields,S.et.al.,(1990)Science249,1046-1049
5.Raycroft,L.etal.,(1990)Science249,1049-1051
6.Hollstein,M.etal.,(1991)Science253,49-53
7.Bennett,W.etal.,(1992)Chest101,19S-20S
8.Xuetal.,(2005)NatCellBio,7:165-171
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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