Excellgen/p53 [1-342] C-terminal deletion, recombinant protein/RP-102/5,000 U,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/p53 [1-342] C-terminal deletion, recombinant protein/RP-102/5,000 U

产品编号：RP-102

市场价：¥6000.00

场地：美国(厂家直采)

产品分类：蛋白类>重组蛋白>其它动物抗原>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$300.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

ProductName	Recombinanthumanp53[1-342]protein(C-terminaldeletion)
Size	5,000U
Description	Thisgeneencodestumorproteinp53,whichrespondstodiversecellularstressestoregulatetargetgenesthatinducecellcyclearrest,apoptosis,senescence,DNArepair,orchangesinmetabolism.p53proteinisexpressedatlowlevelinnormalcellsandatahighlevelinavarietyoftransformedcelllines,whereit'sbelievedtocontributetotransformationandmalignancy.p53isaDNA-bindingproteincontainingtranscriptionactivation,DNA-binding,andoligomerizationdomains.Itispostulatedtobindtoap53-bindingsiteandactivateexpressionofdownstreamgenesthatinhibitgrowthand/orinvasion,andthusfunctionasatumorsuppressor.Mutantsofp53thatfrequentlyoccurinanumberofdifferenthumancancersfailtobindtheconsensusDNAbindingsite,andhencecausethelossoftumorsuppressoractivity.Alterationsofthisgeneoccurnotonlyassomaticmutationsinhumanmalignancies,butalsoasgermlinemutationsinsomecancer-pronefamilieswithLi-Fraumenisyndrome.Multiplep53variantsduetoalternativepromotersandmultiplealternativesplicinghavebeenfound.Thesevariantsencodedistinctisoforms,whichcanregulatep53transcriptionalactivity. Humanwild-typep53proteiniscomposedof393aminoacidresidueswithseveraldistinctregions.TheN-terminalactivationdomainallowsp53proteintorecruitthebasaltranscriptionmachineryandactivatetheexpressionoftargetgenes,whereasthecoredomainbindstotargetDNAinasequence-specificmannerandthemajorityofmutationsfoundinhumantumorsoccurintheregionofthegeneencodingthisdomain(1-3).TheC-terminaldomainiscomposedofpredominantlybasicresiduesandmodificationoftheC-terminalbasicdomain,includingacetylation,glycosylationandphosphorylation,isanessentialmechanismforregulatingp53function(4-6). Thismutantp53protein,withthedeletionoftheC-terminus51residuesincludingtheentirebasicdomainandaportionofthetetramerizationdomain,canbeusedasauniquetooltostudyspecificfunctionsofp53relatedtotheC-terminus.
Applications	gelmobilityshiftassayorforaDNaseIfootprintingassayinthepresenceofdoublestrandedDNAcontainingaconsensusp53-bindingsequence[5’-PuPuPuC(A/T)(T/A)GPyPyPy-3’] invitrotranscriptionassay protein-proteininteractionassay forcellgrowthassay
Source	baculovirus/sf9cells
FusionTag	6XHis
PurificationMethod	FPLC
UnitDefinition(Activity)	1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;50unitsaresufficientforreconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay.
Purity	~95%asdeterminedbySDS-PAGE
GeneID	7157
Accession#	NM_000546
GeneName	tumorproteinp53
GeneSymbol	TP53
GeneSynonyms	P53;BCC7;LFS1;TRP53
MW	39.5kDa
ProteinSequence	EPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDE APRMPEAAPPVAPAPAAPTPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSV TCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAMAIYKQSQHMTEVVRRCPHHERC SDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNSSC MGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPG STKRALPNNTSSSPQPKKKPLDGEYFTLQIRGRERFEMF
StorageBuffer	20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA
Storage	-20to-80°C.
Shipping	4°Cordryice
References	1.Finlay,C.etal.,(1989)Cell57,1083-1093 2.Michalovitz,D.etal.,(1990)Cell62,671-680 3.Baker,S.etal.,(1990)Science249,912-915 4.Fields,S.et.al.,(1990)Science249,1046-1049 5.Raycroft,L.etal.,(1990)Science249,1049-1051 6.Hollstein,M.etal.,(1991)Science253,49-53 7.Bennett,W.etal.,(1992)Chest101,19S-20S 8.Xuetal.,(2005)NatCellBio,7:165-171

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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