| ProductName | VHL(vonHippel-Lindautumorsuppressorprotein) |
| Size | 5,000U |
| Description | vonHippel-Lindau(VHL)diseaseisahereditarycancerwithapredilectionforthecentralnervoussystemandretina(1).ThevonHippel-LindautumorsuppressorgeneismutatedinfamilieswithvonHippel-Lindaudiseaseandencodesaprotein(VHL)of213aminoacidswithanacidicpentapeptidemotifintheN-terminus(2-4).MutationsintheVHLgeneresultinconstitutiveexpressionofmanyhypoxi-inducedgenes,atleastinpartbecauseofincreasesinthecellularlevelofhypoxia-inducIBLetranscriptionfactorHIF-1a(5).VHLproteinbindstoelonginB,elonginC,andCul2toformastablecomplexthattargetshypoxiainducibalfactors(HIFs)fordegradationandtranscriptionalregulation(6,7).Inaddition,VHLproteinhasalsobeenshowntointeractwithspecificproteinkinaseCisoforms(8),histonedeacetylasesandHIF-1inhibitor(FIH-1)(9). |
| Applications |
|
| Source | Baculovirus-insectcell. |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.30-100units(ng)arerequiredforaninvitrotranscriptionassayandprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7428 |
| Accession# | NM_000551 |
| GeneName | vonHippel-Lindautumorsuppressor,E3ubiquitinproteinligase |
| GeneSymbol | VHL |
| GeneSynonyms | RCA1;VHL1;pVHL;HRCA1 |
| MW | 26.0kDa |
| ProteinSequence | MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPR PVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFR DAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDI VRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD |
| StorageBuffer | 20mMTris-HClpH7.9,100mMNaCl,0.2mMEDTA,1mMDTTand20%glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Singh,AD.etal.,(2001)SurveyofOphthalmology46,117-134 2.Latif,F.etal.,(1993)Science260,1317-1320 3.Linehan,WM.etal.,(1995)J.Am.Med.Assoc.273,564-570 4.Duan,DR.etal.,(1995)Proc.Natl.Acad.Sci.USA92,6459-6463 5.Kamura,T.etal.,(2000)Proc.Natl.Acad.Sci.USA97,10430-10435 6.Duan,DR.etal.,(1995)Science269,1402-1406 7.Iwai,K.etal.,(1999)Proc.Natl.Acad.Sci.USA96,12436-12441 8.Pal,S.etal.,(1997)JBiol.Chem.272,27509-27512 9.Mahon,PC.etal.,(2001)Genes&Dev.15,2675-2686 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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