| ProductName | Wilm’sTumorSuppressor1(-KTS) |
| Size | 5,000U |
| Description | WT-1,theproductofWilms’tumorsuppressorgeneWt1,isanuclearproteinwithstructuralmotifscharacteristicoftranscriptionfactors,includingfourC-terminalzincfingers.Whiledifferentpre-mRNAprocessingcouldresultin16isoformsoftheprotein,inclusionorexclusionofexon5andthethreeaminoacids(KTS)betweenzincfingers3and4largelyaffectstheactivityofWT1protein(1-3).Suchacomplexpost-transcriptionalregulation,particularlyinsplicing,mayrepresentamajorregulatorymechanismfortumOrigenesisoftheWilms’tumor.WT1(-KTS)appearstohavedifferentbindingaffinitytobothDNAandRNAcomparingtothe+KTSform(4,5). TheN-terminalFlag-taggedWT-1protein(residue1-446,includingexon5butwithoutKTS)wasexpressedinabaculovirussystemandpurifiedbyaffinityandFPLCchromatography. |
| Applications | invitrofunctionstudiesincluding
|
| Source | Baculovirus-insectcell |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofprotein.2-10unitsaresufficientforagelmobilityshiftassayina20µlreaction.50-200unitsarerequiredforareconstitutedtranscriptionassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 7490 |
| Accession# | NM_000378 |
| GeneName | Wilmstumor1 |
| GeneSymbol | WT-1 |
| GeneSynonyms | GUD;AWT1;WAGR;WT33;NPHS4;WIT-2;EWS-WT1 |
| MW | 55.7kDa |
| ProteinSequence | 1MQDPASTCVPEPASQHTLRSGPGCLQQPEQQGVRDPGGIWAKLGAAEASAERLQGRRSRG 61ASGSEPQQMGSDVRDLNALLPAVPSLGGGGGCALPVSGAAQWAPVLDFAPPGASAYGSLG 121GPAPPPAPPPPPPPPPHSFIKQEPSWGGAEPHEEQCLSAFTVHFSGQFTGTAGACRYGPF 181GPPPPSQASSGQARMFPNAPYLPSCLESQPAIRNQGYSTVTFDGTPSYGHTPSHHAAQFP 241NHSFKHEDPMGQQGSLGEQQYSVPPPVYGCHTPTDSCTGSQALLLRTPYSSDNLYQMTSQ 301LECMTWNQMNLGATLKGVAAGSSSSVKWTEGQSNHSTGYESDNHTTPILCGAQYRIHTHG 361VFRGIQDVRRVPGVAPTLVRSASETSEKRPFMCAYPGCNKRYFKLSHLQMHSRKHTGEKP 421YQCDFKDCERRFSRSDQLKRHQRRHTGVKPFQCKTCQRKFSRSDHLKTHTRTHTGEKPFS 481CRWPSCQKKFARSDELVRHHNMHQRNMTKLQLAL |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Haber,DA.etal.,(1991)Proc.Natl.Acad.Sci.USA88,9618-9622;(1993)Science262,2057-2059 2.Kreidberg,JA.etal.,(1993)Cell74,679-691 3.Larsson,SH.etal.,(1995)Cell81,391-401 4.Little,M.etal.,(1997)Hum.Mutat.9,209-225 5.Englert,C.etal.,(1995)EMBOJ.14,4662-4675 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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