| ProductName | RAD51recombinase |
| Size | 10,000U |
| Description | ProperlycontrolledrecombinationbetweenhomologousDNAmolecules(HomologousRecombination–HR)isessentialforthemaintenanceofgenomestABIlityandforthepreventionoftumOrigenesis.RAD51isamammalianhomologueofyeastRAD51andbacterialRecAand,likeitscounterparts,playsacentralroleinHR.RAD51coatsssDNAtoformanucleoproteinfilamentthatinvadesandpairswithahomologousregioninduplexDNA.ItcanthenactivatestrandexchangetogenerateacrossoverbetweenthejuxtaposedDNAmolecules(1,2).InadditiontoRAD51,thesestepsrequirethecoordinatedactionofanumberofotherhomologous-recombinationproteins,includingtheRP-Aprotein,whichbindssingle-strandedDNA,RAD52,whichcanbindDNAends,annealcomplementarysingle-strandedDNAmoleculesandenhancethespecificityofRAD51,andanumberofRAD51paralogs(3).Thetumour-suppressorproteinsBRCA1andBRCA2colocalizewithRAD51inDNA-damage-inducednuclearfoci(4).BRCA2hasbeenshowntointeractdirectlywithRAD51(5)andthusplaysadirectroleinrepairbyHR,throughcontroloftheavailabilityandfunctionofthecentralmediator,RAD51. |
| Applications | DNAandprotein-proteininteractionsassays |
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.20unitsaresufficientforaDNA-proteinassayand100unitsaresufficientforaprotein-proteininteractionassay. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 5888 |
| Accession# | NM_002875 |
| GeneName | RAD51recombinase |
| GeneSymbol | RAD51 |
| GeneSynonyms | RECA;BRCC5;MRMV2;HRAD51;RAD51A;HsRad51;HsT16930 |
| MW | 39.1kDa |
| ProteinSequence | MAMQMQLEANADTSVEEESFGPQPISRLEQCGINANDVKKLEEAGFHTVEAVAYAPKKEL INIKGISEAKADKILAEAAKLVPMGFTTATEFHQRRSEIIQITTGSKELDKLLQGGIETG SITEMFGEFRTGKTQICHTLAVTCQLPIDRGGGEGKAMYIDTEGTFRPERLLAVAERYGL SGSDVLDNVAYARAFNTDHQTQLLYQASAMMVESRYALLIVDSATALYRTDYSGRGELSA RQMHLARFLRMLLRLADEFGVAVVITNQVVAQVDGAAMFAADPKKPIGGNIIAHASTTRL YLRKGRGETRICKIYDSPCLPEAEAMFAINADGVGDAKD |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.ModestiM.etal.,(2001)GenomeBiol.2(5)Epub 2.AshokRVenkitaraman,(2001)CurrOpCellBiol.13,338-343 3.HiomK.,(2000)CurrBiol9,446-448 4.West,SC,(2003)NatRevMolCellBiol.6,435-445. 5.A.A.Daviesetal.,(2001)MolCell7,273–282 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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