| ProductName | HIF-1aNTAD(Hypoxia-InducIBLeFactor1alphaN-TerminalActivationDomain(530-698) |
| Size | 10,000U |
| Description | Hypoxia-induciblefactor-1(HIF1)isatranscriptionfactorfoundinmammaliancellsculturedunderreducedoxygentensionthatplaysanessentialroleincellularandsystemichomeostaticresponsestohypoxia.HIF1isaheterodimercomposedofanalphasubunitandabetasubunit.Thebetasubunithasbeenidentifiedasthearylhydrocarbonreceptornucleartranslocator(ARNT).ThisgeneencodesthealphasubunitofHIF-1.HIF-1alphacontainstwotransactivationdomainslocatedbetweenaminoacids531and826.Overexpressionofanaturalantisensetranscript(aHIF)ofthisgenehasbeenshowntobeassociatedwithnonpapillaryrenalcarcinomas.Twoalternativetranscriptsencodingdifferentisoformshavebeenidentified.SpecificdisruptionoftheHIF-1pathwayisimportantforexploringitsroleintumorBIOLOGyanddevelopingmoreefficientweaponstotreatcancer(1).HIF-1alphaisamasterregulatorofthehypoxicresponse,anditsproangiogenicactivitiesinclude,butarenotlimitedto,regulationofvascularendothelialgrowthfactor(VEGF)(2).HIF-1alphaproteinexpressionoftenseenininvasivebreastcancer(3).RecentdatademonstratesthatHIF-1alphaknockdownreducestumOrigenicityofMCF-7cellsandsuggestapromisingcombinationofbothanti-HIF-1strategyandtrADItionalchemotherapytoimprovecancertreatment(4) |
| Applications |
|
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 3091 |
| Accession# | NM_001530 |
| GeneName | hypoxiainduciblefactor1,alphasubunit(basichelix-loop-helixtranscriptionfactor) |
| GeneSymbol | HIF1A |
| GeneSynonyms | HIF1;MOP1;PASD8;HIF-1A;bHLHe78;HIF-1alpha;HIF1-ALPHA |
| MW | 19.8kDa |
| ProteinSequence | EFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLAPYIPMDDDFQLRSFDQLSPLESSSASPESASPQSTVTVFQQTQIQEPTANATTTTATTDELKTVTKDRMEDIKILIASPSPTHIHKETTSATSSPYRDTQSRTASPNRAGKGVIEQTEKSHPRSPNVLSVALSQRTT |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Semenza,G.L.etal.,(1994)J.Biol.Chem.269(38),23757-23763 2.Corley,K.M.etal.,(2005)J.Cell.Biochem.96(5),971-985 3.Vleugel,M.M.,etal.,(2005)CancerGenet.Cytogenet.163(2),168-172 4.Li,J.,etal.(2006)Biochem.Biophys.Res.Commun.342(4),1341-1351 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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