| ProductName | Nuclearfactor-like2,NRF2,NFE2L2 |
| Size | 100µg |
| Description | Nuclearfactor(erythroid-derived2)-like2,alsoknownasNFE2L2orNrf2,isatranscriptionfactorthatbindstotheantioxidantresponseelement(ARE)oftargetgenesinresponsetooxidativestressandincreasesthetranscriptionofavarietyofantioxidativeandcarcinogen-detoxificationenzymes.Stresscanresultfromavarietyofcauses,includingfasting,overfeeding,endogenouscompounds,exposuretochemicals,orenvironmentalagents,whichgenerallyleadstoincreasedproductionofreactiveoxygenspecies(ROS).Undernormalconditions,Nrf2isboundtoKelchlike-ECH-associatedprotein1(Keap1).Keap1actsasasubstrateadaptorproteinforCullin3-basedubiquitination,whichresultsinthedegradationofNrf2,andundernormalconditionsNrf2hasahalf-lifeofonly20minutes.OxidativestressorelectrophilicstressdisruptscriticalcysteineresiduesinKeap1,resultinginadisruptionoftheKeap1-Cul3ubiquitinationsystemandabuild-upofNrf2inthecytoplasmandtranslocatestothenucleus,whereitbindstoAREstoinduceexpressionofmultiplecytoprotectiveenzymes,includingNAD(P)H-quinoneoxidoreductase1(NQO1),glutathioneS-transferases(GSTs),andhemeoxygenase1(HO-1)(1,2). Nrf2alsoinducesseveraltranscriptionfactorsinvolvedinmitochondrialbiogenesis.ManylinesofevidencesuggestthatmitochondrialdysfunctionplaysacentralroleinthepathogenesisofPD.Thus,Nrf2maybeapromisingtargettocounteractmitochondrialdysfunctionanditsconsequencesinParkinson'sdisease(PD)(3).TheKeap1–Nrf2regulatorysystemisadouble-edgedswordforourbody.High-levelNrf2activityincreasescellprotectionandmakesthecellresistanttooxidativeandelectrophilicstresses.Therefore,Nrf2inducerscouldbeeffectiveforcancerchemoprevention.Ontheotherhand,elevatedNrf2activityisalsoadvantageousforcancercellsurvivalandevolution.Thus,Nrf2inhibitorscouldbeeffectiveforanticancertherapy.(4) |
| Applications | DNA-protein,protein-proteininteractionresearchandassaydevelopment. |
| Source | E.coli |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 4780 |
| Accession# | NP_006155,NM_006164 |
| GeneName | Nuclearfactor(erythroid-derived2)-like2 |
| GeneSymbol | NFE2L2 |
| GeneSynonyms | NRF2 |
| MW | 69.9kDa |
| ProteinSequence | MMDLELPPPGLPSQQDMDLIDILWRQDIDLGVSREVFDFSQRRKEYELEKQKKLEKERQE QLQKEQEKAFFAQLQLDEETGEFLPIQPAQHIQSETSGSANYSQVAHIPKSDALYFDDCM QLLAQTFPFVDDNEVSSATFQSLVPDIPGHIESPVFIATNQAQSPETSVAQVAPVDLDGM QQDIEQVWEELLSIPELQCLNIENDKLVETTMVPSPEAKLTEVDNYHFYSSIPSMEKEVG NCSPHFLNAFEDSFSSILSTEDPNQLTVNSLNSDATVNTDFGDEFYSAFIAEPSISNSMP SPATLSHSLSELLNGPIDVSDLSLCKAFNQNHPESTAEFNDSDSGISLNTSPSVASPEHS VESSSYGDTLLGLSDSEVEELDSAPGSVKQNGPKTPVHSSGDMVQPLSPSQGQSTHVHDA QCENTPEKELPVSPGHRKTPFTKDKHSSRLEAHLTRDELRAKALHIPFPVEKIINLPVVD FNEMMSKEQFNEAQLALIRDIRRRGKNKVAAQNCRKRKLENIVELEQDLDHLKDEKEKLL KEKGENDKSLHLLKKQLSTLYLEVFSMLRDEDGKPYSPSEYSLQQTRDGNVFLVPKSKKP DVKKN |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.IshiiT.,ItohK.,YamamotoM(2002)RolesofNrf2inactivationofantioxidantenzymegenesviaantioxidantresponsiveelements.MethodsEnzymol348:182–190. |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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