ProductName | ThermusaquaticusMutSDNAmismatchrepairprotein |
Description | TheTaqMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.ThisMutsproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasaThermostableATPaseactivity.ThisthermostableTaqMutSisactiveattemperaturebetween0to75°C.SinceTaqMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.Mutationscanbedetectedinpolyacrylamidegels |
Applications |
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Source | E.coli |
FusionTag | None |
PurificationMethod | FPLC |
Concentration | 1mg/ml |
Purity | ~95%asdeterminedbySDS-PAGE |
Accession# | AAC43637,TAU3311,U33117 |
GeneName | ThermusaquaticusMutSDNAmismatchrepairprotein |
ProteinSequence | 1MEGMLKGEGPGPLPPLLQQYVELRDQYPDYLLLFQVGDFYECFGEDAERLARALGLVLTH 61KTSKDFTTPMAGIPLRAFEAYAERLLKMGFRLAVADQVEPAEEAEGLVRREVTQLLTPGT 121LLQESLLPREANYLAAIATGDGWGLAFLDVSTGEFKGTVLKSKSALYDELFRHRPAEVLL 181APELLENGAFLDEFRKRFPVMLSEAPFEPEGEGPLALRRARGALLAYAQRTQGGALSLQP 241FRFYDPGAFMRLPEATLRALEVFEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHPLLDRG 301PLEARLDRVEGFVREGALREGVRRLLYRLADLERLATRLELGRASPKDLGALRRSLQILP 361ELRALLGEEVGLPDLSPLKEELEAALVEDPPLKVSEGGLIREGYDPDLDALRAAHREGVA 421YFLELEERERERTGIPTLKVGYNAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQRYTLPE 481MKEKEREVYRLEALIRRREEEVFLEVRERAKRQAEALREAARILAELDVYAALAEVAVRY 541GYVRPRFGDRLQIRAGRHPVVERRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQTALIA 601LLAQVGSFVPAEEAHLPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALILKEATENSLVL 661LDEVGRGTSSLDGVAIATAVAEALHERRAYTLFATHYFELTALGLPRLKNLHVAAREEAG 721GLVFYHQVLPGPASKSYGVEVAAMAGLPKEVVARARALLQAMAARREGALDAVLERLLAL 781DPDRLTPLEALRLLQELKALALGAPLDTMKG |
StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,1mMDTT,50%Glycerol |
ReactionBuffer | 100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C |
Storage | -20to-80°C. |
Shipping | 4°Cordryice |
References |
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德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |