Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, C-6X His-MBP-Taq-Muts/EG-197/500 µg,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/Thermus aquaticus Muts DNA mismatch repair protein, C-6X His-MBP-Taq-Muts/EG-197/500 µg

产品编号：EG-197

市场价：¥7900.00

场地：美国(厂家直采)

产品分类：分子类>克隆和表达>转录表达>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$395.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

ProductName	ThermusaquaticusMutSDNAmismatchrepairprotein,C-6XHis-MBP-Taq-Muts
Size	500µg
Description	TheTaqMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.ThisMutsproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasaThermostableATPaseactivity.ThisthermostableTaqMutSisactiveattemperaturebetween0to75°C.SinceTaqMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseoramylosebeads,ormagneticNi-NTAparticles.
Applications	RemovemismatchDNA(errorcorrection)fromgenesynthesisreaction Mutationdetectionandremoval RapidisothermalSNPdetection
Source	E.coli
FusionTag	MBPtagatN-terminusand6XHistagatC-terminus
PurificationMethod	FPLC
Concentration	1mg/ml
Purity	~95%asdeterminedbySDS-PAGE
Accession#	AAC43637,TAU3311,U33117
GeneName	ThermusaquaticusMutSDNAmismatchrepairprotein
MW	135.9kDa
ProteinSequence	1MGKIEEGKLVIWINGDKGYNGLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPD 61IIFWAHDRFGGYAQSGLLAEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYN 121KDLLPNPPKTWEEIPALDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDI 181KDVGVDNAGAKAGLTFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTS 241KVNYGVTVLPTFKGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKP 301LGAVALKSYEEELVKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVD 361EALKDAQTNSSSNNNNNNNNNNLGDDDDKLEVLFQGPHMEGMLKGEGPGPLPPLLQQYVE 421LRDQYPDYLLLFQVGDFYECFGEDAERLARALGLVLTHKTSKDFTTPMAGIPLRAFEAYA 481ERLLKMGFRLAVADQVEPAEEAEGLVRREVTQLLTPGTLLQESLLPREANYLAAIATGDG 541WGLAFLDVSTGEFKGTVLKSKSALYDELFRHRPAEVLLAPELLENGAFLDEFRKRFPVML 601SEAPFEPEGEGPLALRRARGALLAYAQRTQGGALSLQPFRFYDPGAFMRLPEATLRALEV 661FEPLRGQDTLFSVLDETRTAPGRRLLQSWLRHPLLDRGPLEARLDRVEGFVREGALREGV 721RRLLYRLADLERLATRLELGRASPKDLGALRRSLQILPELRALLGEEVGLPDLSPLKEEL 781EAALVEDPPLKVSEGGLIREGYDPDLDALRAAHREGVAYFLELEERERERTGIPTLKVGY 841NAVFGYYLEVTRPYYERVPKEYRPVQTLKDRQRYTLPEMKEKEREVYRLEALIRRREEEV 901FLEVRERAKRQAEALREAARILAELDVYAALAEVAVRYGYVRPRFGDRLQIRAGRHPVVE 961RRTEFVPNDLEMAHELVLITGPNMAGKSTFLRQTALIALLAQVGSFVPAEEAHLPLFDGI 1021YTRIGASDDLAGGKSTFMVEMEEVALILKEATENSLVLLDEVGRGTSSLDGVAIATAVAE 1081ALHERRAYTLFATHYFELTALGLPRLKNLHVAAREEAGGLVFYHQVLPGPASKSYGVEVA 1141AMAGLPKEVVARARALLQAMAARREGALDAVLERLLALDPDRLTPLEALRLLQELKALAL 1201GAPLDTMKGKLAAALEHHHHHH
StorageBuffer	20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol
ReactionBuffer	100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C
Storage	-20to-80°C.
Shipping	4°Cordryice
Protocol (example)	1.AfterfirstroundPCR,purifyPCRfragmentsusingQiagenQIAquickPCRpurificationkitwithelutionindH2O. 2.DilutePCRproductto250ng/µlin10mMTris–HCl,pH7.8,50mMNaClandheatto95^oCfor5minfollowedbycoolingat0.1^oC/sto25^oC. 3.Addbindingbuffer(20mMTris–HCl,pH7.8,10mMNaCl,5mMMgCl₂,1mMDTTand5%glycerol)toannealedPCRproduct,adjustDNAconcentrationto11.5ng/µl,add6XHis-MBP–MutSdimersto950nM. 4.Incubatethemixtureatroomtemperaturefor10min,thenaddanequalvolumeofamyloseresin(NEB)preequilibratedwith1Xbindingbuffer,andincubatefor30minatroomtemperature. 5.Gentlyspindownbeadsandsavesupernatantforsubsequentprocessing(secondroundPCR,cloningetc).
References	1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162. 2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55. 3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4. 4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36. 5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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货号	规格
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Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
Excellgen	Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts	13	EG-266	100 µg
Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">