| ProductName | ThermusThermophilusMutSDNAmismatchrepairprotein |
| Size | 500µg |
| Description | TheTthMutSDNAmismatchproteinrecognizesheteroduplexDNAscontainingmispairedorunpairedbases.This91.8-kDaproteinbindsinvitrotoheteroduplexDNAscontainingmispairedorunpairedbasesoverawidetemperaturerangefrom4to70 °CandhasathermostableATPaseactivity.ThisthermostableTthMutSisactiveattemperaturebetween0to75°C.SinceTthMutSefficientlybindsto1-4basesdeletion(orinsertion)andmismatchbasepairsofGT,CTandAG,itisusefulfordetectingthesemutations.MutationscanbedetectedinpolyacrylamidegelsoronasolidphasesuchasNiagaroseormagneticNi-NTAparticles. |
| Applications |
|
| Source | E.coli |
| FusionTag | 6XHistagatN-terminus |
| PurificationMethod | FPLC |
| Concentration | 1mg/ml |
| Purity | ~95%asdeterminedbySDS-PAGE |
| Accession# | WP_011173381 |
| GeneName | ThermusthermophilusMutSDNAmismatchrepairprotein |
| MW | 135.9kDa |
| ProteinSequence | 1MGSSHHHHHHMGNMLKGEGPGPLPPLLQQYVELRDRYPDYLLLFQVGDFYECFGEDAERL 61ARALGLVLTHKTSKDFTTPMAGIPIRAFDAYAERLLKMGFRLAVADQVEPAEEAEGLVRR 121EVTQLLTPGTLTQEALLPREANYLAAIATGDGWGLAFLDVSTGEFKGTLLKSKSALYDEL 181FRHRPAEVLLAPELRENEAFVAEFRKRFPVMLSEAPFEPQGEGPLALRRAQGALLAYARA 241TQGGALSVRPFRLYDPGAFVRLPEASLKALEVFEPLRGQDTLFGVLDETRTAPGRRLLQA 301WLRHPLLERGPLEARLDRVERFVREGALREGVRRLLFRLADLERLATRLELSRASPRDLA 361ALRRSLEILPELKGLLGEEVGLPDLSGLLEELRAALVEDPPLKVSEGGLIREGYDPDLDA 421LRRAHAEGVAYFLDLEAREKERTGIPTLKVGYNAVFGYYLEVTRPYYEKVPQEYRPVQTL 481KDRQRYTLPEMKERERELYRLEALIKRREEEVFLALRERARKEAEALREAARILAELDVY 541AALAEVAVRHGYTRPRFGERLRIRAGRHPVVERRTAFVPNDLEMAHELVLVTGPNMAGKS 601TFLRQTALIALLAQIGSFVPAEEAELPLFDGIYTRIGASDDLAGGKSTFMVEMEEVALVL 661KEATERSLVLLDEVGRGTSSLDGVAIATALAEALHERRCYTLFATHYFELTALALPRLKN 721LHVAAKEEEGGLVFYHQVLPGPASKSYGVEVAEMAGLPKEVVERARALLSAMAARREGAL 781EEVLERLLALDPDRLTPLEALRFLHELKALALGLPLGSMKG |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,0.1mMEDTA,1mMDTT,50%Glycerol |
| ReactionBuffer | 100mMKCl,50mMTris-HCl,pH8.5,5~20mMMgCl2,0.1mMEDTA,1mMDTT,2%Glycerol,65°C |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Protein-mediatederrorcorrectionfordenovoDNAsynthesis.NucleicAcidsRes.2004Nov23;32(20):e162. 2.CorrectingerrorsinsyntheticDNAthroughconsensusshuffling.NucleicAcidsRes.2005Mar30;33(6):e55. 3.MutSasatoolformutationdetection.ActaBiochimPol.2005;52(3):575-83.Epub2005Aug4. 4.OnetubemutationdetectionusingsensitivefluorescentdyeingofMutSprotectedDNA.NucleicAcidsRes.2000Apr15;28(8):E36. 5.RapidSNPdiagnosticsusingasymmetricisothermalamplificationandanewmismatch-suppressiontechnology.NatureMethods-4,257-262(2007)doi:10.1038/nmeth1007 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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