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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/E. coli RecA/EG-1014/1 mg
产品编号:EG-1014-1mg
市  场 价:¥3960.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$198.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/E. coli RecA/EG-1014/1 mg
商品介绍
Description

RecAisessentialforhomologousrecombination,reactionsinvolvingDNArepairandUV-inducedmutagenesisinEcoli(1,2,3).RecAbindstosinglestrandedDNA,resultinginthepolymerizationofRecAintoanucleoproteincomplex.ThiscomplexcanalignwithcomplementarydoublestrandedDNA,resultinginRecAcatalysisofDNAstrandexchange.RecADNAbindingisstimulatedbyATPhydrolysisornon-hydrolyzableATPanalogs.TheRecA-ATP-singlestrandedDNAcomplexalsocanfunctionasacoproteaseintheproteolyticcleavageofLexA,UmuDandcertainbacteriophageproteins.Inahealthycell,LexArepressestheexpressionofgenesencodingDNArepairproteins(SOSgenes).UponinjuryofDNA,LexAcatalyzesitsowndigestion,therebyallowingsynthesisofnecessarySOSproteins.However,LexAcanonlyinduceself-catalysiswhenactivatedbyassDNA-RecAfilament.AsinglefilamentwillbindandactivateseveralLexAproteins,eachofwhichthencleavesotherboundproteins.Thus,ssDNA-RecA,aproductofDNAinjury,stimulatesDNArepair.

Applications
  • VisualizationofDNAstructureswithelectronmicroscopy(3)
  • D-loopmutagenesis(4)
  • ScreeninglibrariesusingRecA-coatedprobes(5,6)
  • CleavageofDNAatanysinglepredeterminedsite(7,8,9)
  • RecAmediatedaffinitycaptureforfulllengthCDNAcloning(10,11)
SourceAnE.colistrainthatcarriestheE.colirecAgene
Components

RecA:2mg/mlin10mMTris-HCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C

QualityControl

Theabsenceofexo-andendodeoxyribonucleases,non-specificDNaseandRNaseisconfirmedbyappropriatequalitytest.

StorageCondition

-20°C

References

  1. West,S.C.(1992).Ann.Rev.Biochem..61,603-640.
  2. Radding,C.M.(1991).J.Biol.Chem..266,5355-5358.
  3. Wasserman,S.A.andCozzarelli,N.R.(1985).Proc.Natl.Acad.Sci.USA.82,1079-1083.
  4. Shortle,D.etal.(1980).Proc.Natl.Acad.Sci.USA.77,5375-5379.
  5. Honigberg,S.M.etal.(1986).Proc.Natl.Acad.Sci.USA.83,9586-9590.
  6. Rigas,B.etal.(1986).Proc.Natl.Acad.Sci.USA.83,9591-9595.
  7. Ferrin,L.J.andCamerini-Otero,R.D.(1991).Science.254,1494-1497.
  8. Koob,M.etal.(1992).Nucl.AcidsRes..20,5831-5836.
  9. Koob,M.(1992).R.Wu(Ed.),MethodsinEnzymology.216,321-329.SanDiego:AcademicPress.
  10. Zhumabayeva,B.etal.(1990).Biotechniques.27,834-845.
  11. Zhumabayeva,B.etal.(2001).Biotechniques.30,512-520.
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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