Excellgen/T4 Polynucleotide Kinase/EG-1004/1,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品编号：EG-1004-1,000Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA修饰酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	T4PolynucleotideKinase(PNK)catalyzesthetransferoftheterminalgamma-phosphatefromATPtothe5′-OHgroupofsingle-anddouble-strandedDNA,RNA,oligonucleotidesandnucleoside3′-monophosphatemolecules.InthepresenceofADPT4PolynucleotideKinasealsoexhibits5'-phosphataseactivityandcatalyzestheexchangeofphosphategroupsbetween5'-P-oligo-polynucleotidesandATP.
Applications	End-labelingDNAorRNAforprobesandDNAsequencing Additionof5´-phosphatestooligonucleotidestoallowsubsequentligation Removalof3´-phosphorylgroups
Source	ArecombinantE.colistraincarryingT4phagepseTgeneforpolynucleotidekinase.
UnitDefinition	Oneunitisdefinedastheamountofenzymecatalyzingtheincorporationof1nmolof[³²P](ATPdonor)in30minutesat37°Cin1XT4PolynucleotideKinaseReactionBuffer.
Components	T4PolynucleotideKinase:10,000U/mlin10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%Glycerol,0.1μMATP,pH7.4@25°C 10XReactionBuffer:700mMTris-HCl,100mMMgCl₂,50mMDTT,pH7.6@25°C
QualityControl	Theabsenceofendo-,exodeoxyribonucleasesandribonucleasesconfirmedbyappropriatequalitytests.Functionallytestedforlabeling5'-terminiofDNA.
StorageCondition	-20°C

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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