Excellgen/E. coli Exonuclease I/EG-1010/5,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/E. coli Exonuclease I/EG-1010/5,000 Units

产品编号：EG-1010-5,000Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA修饰酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	ExonucleaseIcleavessingle-strandedDNAinthe3'to5'direction,releasing5'-mono/di-nucleotidesandleavingdouble-strandedDNAmoleculesandthe5'-terminusintact.Theenzymeisprocessivethoughdigestionisinhibitedbythepresenceofa3'-terminalphosphate.ThisenzymedoesnotcleaveDNAstrandswithterminal3'-OHgroupsblockedbyphosphoryloracetylgroups
Applications	PrimerremovalfromPCRmixtures: priortoPCRproductsequencing(2) forone-tube"megaprimer"PCRmutagenesis(3) Removalofsingle-strandedDNAcontaininga3'-hydroxylterminusfromnucleicacidmixtures. Assayforthepresenceofsingle-strandedDNAwitha3'-hydroxylterminus(4) IntheprescenceoflamBDaexonuclease,ExoIiseffectiveinremovinglinearDNAfromplasmidpreparations.
Source	AnE.colistrainthatcarriestheclonedEcolisbcBgene
UnitDefinition	Oneunitoftheenzymecatalyzesthereleaseof10nmolofacidsolublenucleotidesin30minat37°C.
MolecularWeight	54.5kDamonomer.
Components	EndonucleaseI:20,000U/mlin10mMTris-HCl,pH7.5@25°C,100mMNaCl,1mMDTT,0.5mMEDTA,50%glycerol 10XReactionBuffer:670mMGlycine-KOH,pH9.5@25°C,67mMMgCl₂,10mMDTT.
QualityControl	Theabsenceofotherendo-,exodeoxyribonucleases,andribonucleasesconfirmedbyappropriatequalitytests.
StorageCondition	-20°C
References	I.R.Lehman,A.L.Nussbaum,ThedeoxyribonucleasesofEscherichiacoli.V.OnthespecificityofexonucleaseI(phosphodiesterase).J.Biol.Chem.239,2628-2636(1964). E.Werleetal.,Convenientsingle-step,onetubepurificationofPCRproductsfordirectsequencing.NucleicAcidsRes.22,4354-4355(1994). S.Nabavi,R.N.Nazar,Simplifiedone-tube“megaprimer”polymerasechainreactionmutagenesis.Anal.Biochem.2,346-348(2005). J.Rosamondetal.,ModulationoftheactionoftherecBCenzymeofEscherichiacoliK-12byCa²⁺.J.Biol.Chem.254,8646-8652(1979). Y.Sasaki,D.Miyoshi,N.Sugimoto,RegulationofDNAnucleasesbymolecularcrowding.NucleicAcidsRes.35,4086-4093(2007).

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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