Excellgen/E coli Exonuclease III/EG-1011/50,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/E coli Exonuclease III/EG-1011/50,000 Units

产品编号：EG-1011-50,000Units

市场价：¥5960.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA修饰酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$298.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	ExonucleaseIII(ExoIII)exhibitsfourcatalyticactivities.The3'=>5'exodeoxyribonucleaseactivityofExoIIIisspecificfordouble-strandedDNA.ExoIIIdegradesdsDNAfrombluntends,5'-overhangsornicks,releases5'-mononucleotidesfromthe3'-endsofDNAstrandsandproducesstretchesofsingle-strandedDNA.Alimitednumberofnucleotidesareremovedduringeachbindingevent,resultingincoordinatedprogressivedeletionswithinthepopulationofDNAmolecules.Itisnotactiveon3'-overhangendsofDNAthatareatleastfour-baseslonganddonotcarrya3'-terminalC-residueonsingle-strandedDNA,oronphosphorothioate-linkednucleotides.Thispropertycanbeexploitedtoproduceunidirectionaldeletionsfromalinearmoleculewithoneresistant(3´-overhang)andonesusceptIBLe(bluntor5´-overhang)terminus. ExoIII3'-phosphataseactivityremovesthe3'-terminalphosphate,generatinga3'-OHgroup.ExoIIIRNaseHactivityexonucleolyticallydegradestheRNAstrandinRNA-DNAhybrids.ExoIIIapurinic/apyrimidinic(AP)endonucleaseactivitycleavesphosphodiesterbondsatapurinicorapyrimidinicsitestoproduce5'-terminithatarebase-freedeoxyribose5'-phosphateresidues.
Applications	CreationofunidirectionaldeletionsinDNAfragmentsinconjunctionwithS1Nuclease. Generationofasingle-strandedtemplatefordideoxy-sequencingofDNA. Site-directedmutagenesis. CloningofPCRproducts. Preparationofstrand-specificprobesfordideoxysequencing
Source	AnE.colistrainthatcarriestheclonedEcolixthgene
UnitDefinition	Oneunitoftheenzymecatalyzesthereleaseof1nmolofacidsolublereactionproductsfromE.coli[³H]-DNAin30minat37°C.
MolecularWeight	31kDamonomer.
Components	EndonucleaseIII:100,000units/mlin25mMTris-HCl,pH8.0@25°C,50mMKCl,1.0mMDTT,0.1%mMEDTA,50%Glycerol 10XReactionBuffer:100mMTris-HCl,pH7.0@25°C,100mMMgCl₂,10mMDTT.
QualityControl	Theabsenceofendodeoxyribonucleasesconfirmedbyappropriatequalitytest. FunctionallytestedforcreationofunidirectionaldeletionsinDNAfragments.
StorageCondition	-20°C
References	S.G.Rogers,B.Weiss,ExonucleaseIIIofEscherichiacoliK-12,anAPendonuclease.MethodsEnzymol.65,201-211(1980). J.D.Hoheisel,OntheActivitiesofEscherichiacoliExonucleaseIII.Anal.Biochem.209,238-246(1993). S.Henikoff,UnidirectionaldigestionwithexonucleaseIIIcreatestargetedbreakpointsforDNAsequencing.Gene.28,351-359(1984). L-H.Guo,R.Wu,NewrapidmethodsforDNAsequencingbasedonexonucleaseIIIdigestionfollowedbyrepairsynthesis.NucleicAcidsRes.10,2065-2084(1982). M.A.Vandeyaretal.,Asimpleandrapidmethodfortheselectionofoligodeoxynucleotide-directedmutants.Gene.65,129-133(1988). C.Li,R.M.Evans,Ligationindependentcloningirrespectiveofrestrictionsitecompatibility.NucleicAcidsRes.25,4165-4166(1997). H.M.Eun,EnzymologyPrimerforRecombinantDNATechnology(AcademicPressInc.,SanDiego,California,1996). J.F.Tomb,G.J.Barcak,Regulatingthe3’-5’activityofexonucleaseIIIbyvaryingthesodiumchlorideconcentration.BioTechniques.7,932-933(1989).

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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