Excellgen/E coli Endonuclease IV (Endo IV), Nfo/EG-160/1,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/E coli Endonuclease IV (Endo IV), Nfo/EG-160/1,000 Units

产品编号：EG-160-1,000Units

市场价：¥1360.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA修饰酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$68.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	EndonucleaseIV(EndoIV)recognizesapurinic/apyrimidinic(AP)sitesofdsDNAandcleavesthephosphodiesterbond5'tothelesiongeneratingahydroxylgroupatthe3'-terminus.Theenzymecanalsoactasa3'-diesterasethatisabletorelease3'-phosphoglycolateor3'-phosphatefromthedamagedendsofdsDNA.EndoIVpossessesalsoa3'=>5'exonucleaseactivity.Itsprogressiononsubstratesissensitivetoionicstrength,metalions,EDTA,andreducingconditions.Substrateswith3'-recessedendsarepreferredsubstratesforthe3'=>5'exonucleaseactivity.
Applications	StudiesofDNAdamageandrepair Singlecellelectrophoresis(cometassay) Antitumordrugresearch DNAstructureresearch SNPanalysis DNADetectionUsingRecombinasePolymeraseAmplification(RPA)
Source	AnE.colistrainwhichcarriestheclonedEndonucleaseIV(nfo)gene
UnitDefinition	Oneunitisdefinedastheamountofenzymerequiredtocleave1pmolofa34-meroligonucleotideduplexcontainingasingleAPsiteinatotalreactionvolumeof10µlin1hourat37°C. AnAPsiteiscreatedbytreating10pmolofa34meroligonucleotideduplexcontainingasingleuracilresiduewith1unitofUracil-DNAGlycosylase(UDG)for2minutesat37°C.
Components	EndonucleaseIV:10,000U/mlinstoragebuffercontaining50mMTris-HCl(pH7.5),0.1MNaCl,1mMDTT,0.05%(v/v)TritonX-100,and50%(v/v)glycerol. 10XReactionBuffer:1000mMNaCl,500mMTris-HCl,100mMMgCl2,10mMDTT,pH7.9@25°C
QualityControl	Theabsenceofotherendo-,exodeoxyribonucleases,andribonucleasesconfirmedbyappropriatequalitytests.
StorageCondition	-20°C
References	J.D.Levin,HomogeneousEscherichiacoliendonucleaseIV.CharacterizationofanenzymethatrecognizesoxidativedamageinDNA.J.Biol.Chem.263,8066-8071(1988). S.M.Kerins,etal.,CharacterizationofanendonucleaseIV3’-5’exonucleaseactivity.J.BiolChem.278(5),3048-3054(31January2003). B.Demple,L.Harrison,RepairofoxidativedamagetoDNA:enzymologyandBIOLOGy.Annu.Rev.Biochem.63,915-948(1994). J.D.Levin,B.Demple,InvitrodetectionofendonucleaseIV–specificDNAdamageformedbybleomycininvivo.NucleicAcidsRes.24,885-889(1996) J.N.Patro,etal.,ProbingtheconfigurationsofformamidopyrimidinelesionsFapy.dAandFapy.dGinDNAusingendonucleaseIV.Biochemistry.43,13397-13403(2004). A.R.Collins,ThecometassayforDNAdamageandrepair:principles,applications,andlimitations.Molec.Biotechnol.26,249-261(2004). D.J.Hosfieldetal.,StructureoftheDNArepairenzymeendonucleaseIVanditsDNAcomplex:double-nucleotideflippingatabasicsitesandthree-metal-ioncatalysis.Cell.98,397-408(1999). I.V.Kutyavinetal.,AnovelendonucleaseIVpost-PCRgenotypingsystem.NucleicAcidsRes.29,1-9(2006).

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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