| Description | FLPrecombinasecatalyzesthesite-specificrecombinationofDNAbetweenFRTsites.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem. Recombinantcell-permeantFLPrecombinaseTat-FlpwaspurifiedfromanHEK293celllineoverexpressingFLPRecombinasefromyeastSaccharomycescerevisiaewithadditionalN-terminal6XHistag,aTatpeptide(GRKKRRQRRRPPAGTSVSL)andanNLSsequence(PKKKRKV).Inaddition,this"Thermostable"mutantFLP(P2SL33SY108NS294P)has~4-10foldhigheractivitythanwild-typeFLPat37°C. |
| Applications | exvivoFRTrecombination. |
| QC | Additionof100unitsofTat-FlptoFRT-RFP-Stop-FRT-GFP293Treportercellsresultedin60~90%recombinationefficiency.  Figure1:Tat-Flptransductionefficiencyisconcentrationdependent. 1:noTat-FLP;2~8:adding5,10,25,50,75,100and200unitsofTat-FLPtoHEK293TcellstransfectedwithFRT-RFP-Stop-FRT-GFPplasmidDNA.Picturesweretaken4daysaftertransductionwithvariousamountofTat-FLP |
| UnitDefinition | Astandardof100UnitsisdefinedastheamountofTAT-FLPin1.0mLoftissueculturemediumthatisrequiredtoinduce50% GFPexpressioninaHEK293Treportercelllineassay. |
| Source | Sf9cells |
| Storage | -20~-80°C. |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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