| Description | RecombinantCrerecombinase(TAT-Cre)waspurifiedfromanHEK293celllineexpressingenhancedformofCreRecombinasefrombacteriophageP1.ThisCrerecombinasehasanN-terminal6XHistag,aTatpeptide(GRKKRRQRRRPPAGTSVSL)andanNLSsequence(PKKKRKV).Tat-Creisthemosteffectiveproteinintransduction(invivo)andsubsequentrecombinationcomparedtootherformsofCrerecombinases,e.g.,HNC,TCH6,HC,HNCM,CH. |
Applications |
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| Source | HEK293cells |
| Activity | Astandardof100UnitsisdefinedastheamountofTat-Crein1.0mLoftissueculturemediumthatisrequiredtoinduce90%GFPexpressioninaHEK293TloxPreportercelllineassay. |
| Properties | QC:HEK293T-Crereportercellline80~100%recombinationefficiencies.  Figure1.QCassayofTat-Cre.Adding 50and100unitsofTat-CretoHEK293TcellstransfectedwithLoxP-RFP-Stop-LoxP-GFPreporterplasmidDNA. EndotoxinLevels:<0.1EU/ug |
| Storage | -20ºC~-80ºC |
| Shipping | dryice |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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