Excellgen/T3 DNA Ligase/EG-1043/100,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/T3 DNA Ligase/EG-1043/100,000 Units

产品编号：EG-1043-100,000Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>连接酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	T3DNALigaseisanATP-dependentdsDNAligasefrombacteriophageT3.Itcatalyzestheformationofaphosphodiesterbondbetweena5′phosphateanda3′hydroxylterminiinduplexDNA.T3DNALigasecanefficientlycatalyzeligationofbothcohesiveendsandbluntendsandnicksealing.T3DNALigaseis5-foldmoreefficientthanT4DNAligaseforligationofcohesiveDNAfragments,butnotasactiveasT4DNAligaseforblunt-endedDNAfragments,althoughthebluntendligationcanbeenhancedbytheadditionof15%PEG6000tothereaction.T3DNALigasedisplaysahigherefficiencyforjoiningA/ToverhangsthanC/Gmatchedends,thereforeitcanbeusedforTAcloning.CohesiveligationwithT3DNALigasecanbecompletedinlessthan10min.
Applications	CloningofDNAfragmentsgeneratedbyrestrictionenzymedigestion AddinglinkersoradapterstodsDNA CircularizationoflinearDNA Nick-sealingindsDNA Site-directedmutagenesis GoldenGateassemblyofDNAfragmentssuchasTranscriptionActivator-LikeEffectorNucleases(TALEN)
Source	PurifiedfromarecombinantE.colistraincarryingtheT3DNALigasegene
Components	T3DNALigase:3,000,000U/mlinstoragebuffer:20mMTris-HCl,50mMNaCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C. 4XT3DNALigationBuffer:250mMTris-HCI,40mMMgCl₂,4mMDTT,4mMATP,30%PEG6000,pH7.6@25°C
UnitDefinition	1unitisdefinedastheamountofT3DNALigaserequiredtoligate50%of100ngHindIIIDNAfragmentswithcohesiveterminiin30minutesat23°C
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
Excellgen	GeneExpresso™Max Transfection Reagent	2	EG-1089	1ml
Excellgen	Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)	4	EG-1069	1 mg
Excellgen	Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg
Excellgen	serine/threonine kinase 33, STK33	7	EG-261	100 µg
Excellgen	Alpha-tubulin Rabbit Polyclonal Antibody	9	EG-1000	100 µg
Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
Excellgen	Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts	13	EG-266	100 µg
Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">