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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/T7 DNA Ligase/EG-1033/1,000,000 Units
产品编号:EG-1033-1,000,000Units
市  场 价:¥5000.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>连接酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$250.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/T7 DNA Ligase/EG-1033/1,000,000 Units
商品介绍
Description

T7DNALigaseisanATP-dependentdsDNAligasefrombacteriophageT7.Itcatalyzestheformationofaphosphodiesterbondbetweena5′phosphateanda3′hydroxylterminiinduplexDNA.CohesiveendligationandnicksealingcanbeefficientlycatalyzedbyT7DNALigase.However,itislessefficientthanT4andT3DNALigasesforbluntendligationCohesiveligationwithT7DNALigasecanbecompletedinlessthan10minutes

Applications
  • CloningofDNAfragmentsgeneratedbyrestrictionenzymedigestion
  • AddinglinkersoradapterstodsDNA
  • CircularizationoflinearDNA
  • Nick-sealingindsDNA
  • Site-directedmutagenesis
  • GoldenGateassemblyofDNAfragmentssuchasTranscriptionActivator-LikeEffectorNucleases(TALEN)
SourcePurifiedfromarecombinantE.colistraincarryingtheT7DNALigasegene
Components
  • T7DNALigase,3,000,000U/mlinstoragebuffer:20mMTris-HCl,50mMNaCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C.
  • 4XT7DNALigationBuffer:250mMTris-HCI,40mMMgCl2,4mMDTT,4mMATP,30%PEG6000,pH7.6@25°C
UnitDefinition1unitisdefinedastheamountofT7DNALigaserequiredtoligate50%of100ngDNAfragmentswithcohesiveterminiin30minutesat23°C
QualityControl
  • Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage-20°C.
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




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2

EG-1089

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4

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Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




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serine/threonine kinase 33, STK33

7

EG-261

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9

EG-1000

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11

EG-156

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13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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