Excellgen/Chlorella Virus PBCV-1 DNA Ligase/EG-1063/1,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/Chlorella Virus PBCV-1 DNA Ligase/EG-1063/1,000 Units

产品编号：EG-1063-1,000Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>连接酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	ChlorellavirusPBCV-1DNAligase(ChlorellavirusDNAligase,alsoknownasSplintR™LigasesoldbyNEB),isanATP-dependentDNAligase.PBCV-1DNAligaseefficientlycatalyzestheligationofadjacent,single-strandedDNAsplintedbyacomplementaryRNAstrand.ThisunusualactivitycanbeusedtocharacterizemiRNAs,RNAsandSNPs,e.g.sensitive(sub-nanomolarrange)detectionofspecificRNAbyligation.Inaddition,PBCV-1DNAligasemaybeusedforenrichmentofRNAfornext-generationsequencing. PBCV-1DNAligaseenzymeisactiveoverabroadrangeofATPconcentrations(10µM–1mM)andpH(6.5–9).OptimalactivityisobservedusingMg2+>5mM,andapHbetween7.5and8.0.Theactivityisenhancedatelevatedtemperatures(upto37°C),andbysupplementationwith5mMMn2+.Thereactionisinhibitedbysaltconcentrationswhenconcentrationis>100mM. Theenzymetoleratesallbasepaircombinationsattheligationjunction,butispartiallyinhibitedbydC/GanddG/Cbasepairsatthedonor(phosphorylated)sideligationjunction,particularlywhenthe+2basewasalsoaC/Gbasepair.
Applications	LigationofssDNAsplintedbycomplementaryRNAsequences DetectionofRNAusingligationofDNAprobes SNPorsplicevariantdetection RASL-seq
Source	PurifiedfromarecombinantE.colistraincarryingthePBCV-1DNAligasegene
Components	ChlorellavirusPBCV-1DNAligase,10,000unitsinstoragebuffer:10mMTris-HCl,300mMNaCl,1mMDTT,0.1mMEDTA,50%Glycerol,pH7.4@25°C 10XLigationBuffer:250mMTris-HCI,50mMMgCl₂,5mMDTT,10mMATP,pH7.6@25°C
UnitDefinition	Oneunitisdefinedastheamountofenzymeneededtoligate(to50%completion)2picomolesofatripartiteFAM-labeledDNA:RNAhybridsubstrateina20μlreactionat25°Cin15minutesin1XLigationBuffer.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,andribonucleasesconfirmedbyappropriatequalitytests.
Storage	-20°C.

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
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Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">