请使用支持JavaScript的浏览器!
主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/T4 RNA Ligase 2, dsRNA Ligase/EG-1052/250 Units
产品编号:EG-1052-250Units
市  场 价:¥1000.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>连接酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$50.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/T4 RNA Ligase 2, dsRNA Ligase/EG-1052/250 Units
商品介绍
DescriptionT4RNALigase2,alsoknownasT4Rnl2(gp24.1),hasbothintermolecularandintramolecularRNAstrandjoiningactivity.T4RNALigase2ismuchmoreactivejoiningnicksondoublestrandedRNAthanonjoiningtheendsofsinglestrandedRNA.Ligationofsingle-strandedRNAsubstratesgenerateseitherintramolecularorintermolecularproducts.Theenzymerequiresanadjacent5´phosphateand3´OHforligation.Theenzymecanalsoligatethe3´OHofRNAtothe5´phosphateofDNAinadoublestrandedstructure.Thenon-ligatedstrandoftheduplexcanbeeitherRNAorDNA.T4RNAligase2requiresATPforactivityunlessthesubstrateispreadenylatedonthe5′end.
Applications
  • LigateanickindsRNA
  • Ligatethe3´OHofRNAtothe5´phosphateofDNAinadoublestrandedstructure
Source

PurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2(gp24.1)gene

Components
  • T4RNALigase2:30,000U/mlinstoragebuffer:10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C
  • 10XT4RNALigase2Buffer:500mMTris-HCl,100mMMgCl2,50mMDTT,10mMATP,pH7.6@25°C
UnitDefinition

Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′phosphorylatedendofa18-merDNAwhenbothstrandsareannealedtoacomplementary35-merDNAstrandin20µLat37°Cfor30minutes.

QualityControl
  • Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage-20°C.
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

<span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">


自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔