Excellgen/T4 RNA Ligase 2, dsRNA Ligase/EG-1052/250 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品编号：EG-1052-250Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>连接酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	T4RNALigase2,alsoknownasT4Rnl2(gp24.1),hasbothintermolecularandintramolecularRNAstrandjoiningactivity.T4RNALigase2ismuchmoreactivejoiningnicksondoublestrandedRNAthanonjoiningtheendsofsinglestrandedRNA.Ligationofsingle-strandedRNAsubstratesgenerateseitherintramolecularorintermolecularproducts.Theenzymerequiresanadjacent5´phosphateand3´OHforligation.Theenzymecanalsoligatethe3´OHofRNAtothe5´phosphateofDNAinadoublestrandedstructure.Thenon-ligatedstrandoftheduplexcanbeeitherRNAorDNA.T4RNAligase2requiresATPforactivityunlessthesubstrateispreadenylatedonthe5′end.
Applications	LigateanickindsRNA Ligatethe3´OHofRNAtothe5´phosphateofDNAinadoublestrandedstructure
Source	PurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2(gp24.1)gene
Components	T4RNALigase2:30,000U/mlinstoragebuffer:10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C 10XT4RNALigase2Buffer:500mMTris-HCl,100mMMgCl₂,50mMDTT,10mMATP,pH7.6@25°C
UnitDefinition	Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′phosphorylatedendofa18-merDNAwhenbothstrandsareannealedtoacomplementary35-merDNAstrandin20µLat37°Cfor30minutes.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
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