Excellgen/T4 RNA Ligase 2 Truncated/EG-1053/2,500 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/T4 RNA Ligase 2 Truncated/EG-1053/2,500 Units

产品编号：EG-1053-2,500Units

市场价：¥1000.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>连接酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$50.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	T4RNALigase2truncatedcatalyzesphosphodiesterbondformationbetweenapre-adenylated5′phosphate(DNAorRNA)andthe3′hydroxylofRNA.Thetruncatedenzymecontainingthefirst249aminoacidsisunabletoadenylatethe5´endofthesubstrate,thusitcannotligatethephosphorylated5´endofRNAorDNAtothe3´endofRNA.Suchapropertyisusefulforcertainapplicationssuchaslinkerligationswithpre-adenylated5′DNAto3′hydroxylRNA,suchasmicroRNAs.Thistruncatedenzymesignificantlyreducesunwantedbackgroundligationasitcanonlyuseadenylatedprimers.
Applications	Ligateapre-adenylatedDNAorRNAsequencetagtoanyRNA3´-end JoinasinglestrandedadenylatedprimertosmallRNAsforCDNAlibrarycreation JoinasinglestrandedadenylatedprimertoRNAforstrand-specificcDNAlibraryconstruction
Source	PurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2Rnl2(gp24.1)(1-249)gene
Components	T4RNALigase2truncated:200,000units/mlinstoragebuffer:10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C 10XT4RNALigase2truncatedBuffer:500mMTris-HCl,100mMMgCl₂,50mMDTT,pH7.6@25°C
UnitDefinition	Oneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′pre-adenylatedendofa18-merDNAwhenboth17-mersareannealedtoacomplementary35-merDNAstrandin20μL1Xreactionbufferfollowinga30minuteincubationat37°C.
QualityControl	Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage	-20°C.

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
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