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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/T4 RNA Ligase 2 Truncated/EG-1053/2,500 Units
产品编号:EG-1053-2,500Units
市  场 价:¥1000.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>连接酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$50.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/T4 RNA Ligase 2 Truncated/EG-1053/2,500 Units
商品介绍
DescriptionT4RNALigase2truncatedcatalyzesphosphodiesterbondformationbetweenapre-adenylated5′phosphate(DNAorRNA)andthe3′hydroxylofRNA.Thetruncatedenzymecontainingthefirst249aminoacidsisunabletoadenylatethe5´endofthesubstrate,thusitcannotligatethephosphorylated5´endofRNAorDNAtothe3´endofRNA.Suchapropertyisusefulforcertainapplicationssuchaslinkerligationswithpre-adenylated5′DNAto3′hydroxylRNA,suchasmicroRNAs.Thistruncatedenzymesignificantlyreducesunwantedbackgroundligationasitcanonlyuseadenylatedprimers.
Applications
  • Ligateapre-adenylatedDNAorRNAsequencetagtoanyRNA3´-end
  • JoinasinglestrandedadenylatedprimertosmallRNAsforCDNAlibrarycreation
  • JoinasinglestrandedadenylatedprimertoRNAforstrand-specificcDNAlibraryconstruction
SourcePurifiedfromarecombinantE.colistraincarryingtheT4phageRNALigase2Rnl2(gp24.1)(1-249)gene
Components
  • T4RNALigase2truncated:200,000units/mlinstoragebuffer:10mMTris-HCl,100mMNaCl,0.1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C
  • 10XT4RNALigase2truncatedBuffer:500mMTris-HCl,100mMMgCl2,50mMDTT,pH7.6@25°C
UnitDefinitionOneunitisdefinedastheamountofenzymerequiredtoligate50%of0.4µgofanequimolarmixofasingle-stranded5′FAM-labeled17-merRNAtothe5′pre-adenylatedendofa18-merDNAwhenboth17-mersareannealedtoacomplementary35-merDNAstrandin20μL1Xreactionbufferfollowinga30minuteincubationat37°C.
QualityControl
  • Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojoincohesive-andblunt-endDNAfragments.
Storage-20°C.
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

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货号

规格




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13

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15

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100 µg

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