Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/100 μg,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/100 μg

产品编号：EG-42

市场价：¥3960.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>连接酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$198.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	SortaseAisabifunctionalproteindisplayingbothproteinligase/transpeptidaseandcysteineproteaseactivities.Theligaseacitivityallowsproteinligationgeneratingfusionproteinsornon-peptidicbioconjugates,suchasunnaturalaminoacids,nucleicacids,fluorophores,chemicaldyesandligands(biotin),antibiotics,sugarderivatives,etc.TheproteaseactivityofsortaseAfacilitatesthecleavageoffusionproteinsjoinedbyLPXTGGmotifs.Thecleavageproductsserveassubstratesforproteinligation.Consequently,sortaseAmediatesreactionsreachanassay-specificequilibriumbetweenligationandcleavageactivities.
Applications	invitroproteinligation:ligateofLPETGG-taggedtargetproteintogycinemodifiedoligonucleotideorGGK-fluorophores Immobilizationofproteinstosolidsurfaces Cell-surfacelabellingoflivingorganisms Proteincircularization Posttranslationalincorporationofnovelfunctionalityinproteins CleavageoffusionproteinsjoinedbyLPXTGGmotifs.
Source	PurifiedfromarecombinantE.colistraincarryingtheStaphylococcusaureussortaseAaminoacids26–206.
Components	SortaseA,1mg/mlinstoragebuffer:10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C.
Enzymaticactivity	Sortasereactionscannotproceedwith100%efficiency,neitherwithrespecttoproteinligase,nortoproteaseactivity.Ratheranassayspecificequilibriumbetweenligatedandcleavedproductisobtained.Inconsequence,sortaseassayswillalwaysyieldamixtureofcleavedandligatedproteins.Thus,furtherpurificationstepsareneededtopurifyfusionprotein.
QualityControl	Theabsenceofendo-,exodeoxyribonucleasesconfirmedbyappropriatequalitytests. Functionallytestedforthecapacitytojointwoproteins
Storage	-20°C.

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
Excellgen	GeneExpresso™Max Transfection Reagent	2	EG-1089	1ml
Excellgen	Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)	4	EG-1069	1 mg
Excellgen	Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg
Excellgen	serine/threonine kinase 33, STK33	7	EG-261	100 µg
Excellgen	Alpha-tubulin Rabbit Polyclonal Antibody	9	EG-1000	100 µg
Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
Excellgen	Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts	13	EG-266	100 µg
Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">