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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/100 μg
产品编号:EG-42
市  场 价:¥3960.00
场      地:美国(厂家直采)
产品分类: 分子类>核酸酶类>连接酶>
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$198.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/Bifunctional Protein Ligase/Protease Sortase A/EG-42/100 μg
商品介绍
DescriptionSortaseAisabifunctionalproteindisplayingbothproteinligase/transpeptidaseandcysteineproteaseactivities.Theligaseacitivityallowsproteinligationgeneratingfusionproteinsornon-peptidicbioconjugates,suchasunnaturalaminoacids,nucleicacids,fluorophores,chemicaldyesandligands(biotin),antibiotics,sugarderivatives,etc.TheproteaseactivityofsortaseAfacilitatesthecleavageoffusionproteinsjoinedbyLPXTGGmotifs.Thecleavageproductsserveassubstratesforproteinligation.Consequently,sortaseAmediatesreactionsreachanassay-specificequilibriumbetweenligationandcleavageactivities.
Applications
  • invitroproteinligation:ligateofLPETGG-taggedtargetproteintogycinemodifiedoligonucleotideorGGK-fluorophores
  • Immobilizationofproteinstosolidsurfaces
  • Cell-surfacelabellingoflivingorganisms
  • Proteincircularization
  • Posttranslationalincorporationofnovelfunctionalityinproteins
  • CleavageoffusionproteinsjoinedbyLPXTGGmotifs.
SourcePurifiedfromarecombinantE.colistraincarryingtheStaphylococcusaureussortaseAaminoacids26–206.
Components
  • SortaseA,1mg/mlinstoragebuffer:10mMTris-HCl,50mMKCl,1mMDTT,0.1mMEDTA,50%glycerol,pH7.5@25°C.
EnzymaticactivitySortasereactionscannotproceedwith100%efficiency,neitherwithrespecttoproteinligase,nortoproteaseactivity.Ratheranassayspecificequilibriumbetweenligatedandcleavedproductisobtained.Inconsequence,sortaseassayswillalwaysyieldamixtureofcleavedandligatedproteins.Thus,furtherpurificationstepsareneededtopurifyfusionprotein.
QualityControl
  • Theabsenceofendo-,exodeoxyribonucleasesconfirmedbyappropriatequalitytests.
  • Functionallytestedforthecapacitytojointwoproteins
Storage-20°C.
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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