| ProductName | ThyroidHormoneReceptorbeta1isoform |
| Size | 10,000U |
| Description | Nuclearreceptorsformthelargestknownfamilyoftranscriptionfactorsandhaveacrucialroleinnearlyallaspectsofvertebratedevelopmentandadultphysiologybytransducingtheeffectsofhormonesintotranscriptionalresponses(1).Thefamilyisdefinedbytwodomains:(a)thecentral,highlyconserved,DNA-bindingdomain(DBD)ofapproximately66aminoacids,and(b)theC-terminal,structurallyconserved,ligand-bindingdomain(LBD)ofapproximately250aminoacids(2,3).Theamino-terminalregionsareleastconservedamongnuclearreceptorsequences.ThisdomainishighlydivergentbetweenTRαandTRβisoforms,whichsuggestsdifferentialrolesintranscriptionalregulation.Inaddition,alternativesplicingoftheTRαgenegeneratestwoisoforms,TRα1andTRα2withcompletelydifferentamino-terminaldomains(4).UnligandedTRinhibitstheformationofafunctionalpre-initiationcomplex,throughdirectinteractionwithTBPandtranscriptionfactorIIB(5-7).Inaddition,intheabsenceofligand,TRhasbeenshowntorepresstranscriptionthroughrecruitmentofacorepressorcomplex,whichalsoincludesSin3Aandhistonedeacetylase(8,9).Ligandbindingreleasesthecorepressorcomplexandrecruitsacoactivatorcomplexthatincludesmultiplehistoneacetyltransferases,includingasteroidreceptorfamilycoactivator,p300/CREB-bindingprotein–associatedfactor(PCAF),andCREBbindingprotein(CBP)(10-14). |
| Applications |
|
| Source | E.coli |
| FusionTag | Histag |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 6257 |
| Accession# | NM_021976 |
| GeneName | retinoidXreceptor,beta |
| GeneSymbol | RXRB |
| GeneSynonyms | NR2B2;DAUDI6;RCoR-1;H-2RIIBP |
| MW | 58.6kDa. |
| ProteinSequence | MTPNSMTENGLTAWDKPKHCPDREHDWKLVGMSEACLHRKSHSERRSTLKNEQSSPHLIQ TTWTSSIFHLDHDDVNDQSVSSAQTFQTEEKKCKGYIPSYLDKDELCVVCGDKATGYHYR CITCEGCKGFFRRTIQKNLHPSYSCKYEGKCVIDKVTRNQCQECRFKKCIYVGMATDLVL DDSKRLAKRKLIEENREKRRREELQKSIGHKPEPTDEEWELIKTVTEAHVATNAQGSHWK QKRKFLPEDIGQAPIVNAPEGGKVDLEAFSHFTKIITPAITRVVDFAKKLPMFCELPCED QIILLKGCCMEIMSLRAAVRYDPESETLTLNGEMAVTRGQLKNGGLGVVSDAIFDLGMSL SSFNLDDTEVALLQAVLLMSSDRPGLACVERIEKYQDSFLLAFEHYINYRKHHVTHFWPKLLMKVTDLRMIGACHASRFLHMKVECPTELFPPLFLEVFED |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,1mMDTT,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Mangelsdorf,D.J.,etal.,(1995)Cell83,835–839 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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