| ProductName | Farnesoid-X-activatedReceptor,FarnesoidXreceptor |
| Size | 10,000U |
| Description | Farnesoid-X-receptor(FXR)wasoriginallyidentifiedandclonedinratasanorphannuclearhormonereceptorbasedonhybridizationwithadegenerateoligonucleotidedesignedfromthehighlyconservednuclearhormonereceptorDNAbindingdomain(1).FXRfunctionsasaheterodimerwithRXRandbindstosequenceelementsinthepromotersoftargetgenes.TheFXR/RXRheterodimerbindswithhighestaffinitytoinvertedrepeatsseparatedby1bp(IR-1)andwithlowaffinitytodirectrepeatsseparatedby4and5bp(DR-4andDR-5)(1,2).Asisthecaseforothernuclearhormonereceptors,FXRregulatestargetgeneactivityinresponsetoligand.WhileinitialstudiessuggestedthatfarnesolandretinoidmetaboliteswerelikelyligandsforFXR,currentdatasupportthenotionthatFXRisabileacidsensorthatplaysanintegralroleinbileacidsynthesisandtransport(1,3-6).Inthesmallintestine,FXRregulatesbileaciduptakethroughtheupregulationoftheilealbileacidbindingproteingeneviabindingtoanupstreamresponseelement(7).TheFXR/RXRheterodimercanbeactivatedbythebilesaltchenodeoxycholicacid(CDCA)andFXRisrequiredforthebilesalt-dependenttranscriptionalcontrolofthehumanABCB11gene(thebilesaltexportpump)(8).Inaddition,FXRhasbeenshowntoinhibitthecholesterol7-hydrolasegene(CYP7A1)transcription(9). |
| Applications | FXRhasbeenappliedinDNAandprotein-proteininteractionsassays |
| Source | E.coli |
| FusionTag | HisTag |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogrampurifiedprotein.20unitsaresufficientforagel-mobilityshiftassayand100unitsaresufficientforaprotein-proteininteractionassay |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 9971 |
| GeneName | FXR |
| GeneSynonyms | BAR;FXR;HRR1;HRR-1;RIP14 |
| MW | 56.6kDa |
| ProteinSequence | MGSKMNLIEHSHLPTTDEFSFSENLFGVLTEQVAGPLGQNLEVEPYSQYSNVQFPQVQPQ ISSSSYYSNLGFYPQQPEEWYSPGIYELRRMPAETLYQGETEVAEMPVTKKPRMGASAGR IKGDELCVVCGDRASGYHYNALTCEGCKGFFRRSITKNAVYKCKNGGNCVMDMYMRRKCQ ECRLRKCKEMGMLAECLLTEIQCKSKRLRKNVKQHADQTVNEDSEGRDLRQVTSTTKSCR EKTELTPDQQTLLHFIMDSYNKQRMPQEITNKILKEEFSAEENFLILTEMATNHVQVLVE FTKKLPGFQTLDHEDQIALLKGSAVEAMFLRSAEIFNKKLPSGHSDLLEERIRNSGISDE YITPMFSFYKSIGELKMTQEEYALLTAIVILSPDRQYIKDREAVEKLQEPLLDVLQKLCK IHQPENPQHFACLLGRLTELRTFNHHHAEMLMSWRVNDHKFTPLLCEIWDVQ |
| StorageBuffer | 20mMTris-HCl,pH8.0,250mMNaCl,50%Glycerol |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Formanetal.,(1995)Cell81,687-693 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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