Excellgen/ExcellScript Thermostable Reverse Transcriptase/EG-1029/100,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/ExcellScript Thermostable Reverse Transcriptase/EG-1029/100,000 Units

产品编号：EG-1029-100,000Units

市场价：¥4760.00

场地：美国(厂家直采)

产品分类：分子类>克隆和表达>克隆基因>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$238.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description

ExCellscript ThermostableM-MuLVReverseTranscriptaseisaDNApolymerasewhichutilizesRNAasasubstrateandexhibitsnomeasurableproofreADIng3′→5′exonucleasefunction.ThisthermostablereversetranscriptasecanperformCDNAsynthesisreactionsthroughoutawiderangeoftemperaturesfrom42°Cto72°C.

Applications

FirststrandcDNAsynthesisforRT-PCRandreal-timeRT-PCR
SynthesisofcDNAforcloningandexpression
GenerationoflabeledcDNAprobesformicroarrays
DNAlabeling
AnalysisofRNAbyprimerextension
IsothermalnucleicacidamplificationsuchasLAMP

Source

ArecombinantE.colistraincarryinganengineeredMoloney-MurineLeukemiaVirusReverseTranscriptasegene

QualityControl

Theabsenceofendo-,exodeoxyribonucleases,phosphatases,andribonucleasesconfirmedbyappropriatequalitytests.
FunctionallytestedinfirststrandcDNAsynthesis.

Unitdefinition

1unitisdefinedastheamountofenzymerequiredtoincorporate1nmolofdTTPintoacidinsolublematerialin10minutesat37°Cusingpolyr(A)/oligo(dT)asasubstrate.

Components

ThermostableM-MuLVReverseTranscriptase:200units/µLin50mMTris-HCl,150mMNaCl,0.1mMEDTA,1mMDTT,0.1%NP-40,50%glycerol,pH7.6@25°C
10XReactionBuffer:500mMTris-HCl,750mMKCl,30mMMgCl₂,100mMDTTpH8.3@25°C

Protocol

PrimerAnnealing:CombinethefollowinginanRNase-freereactionvessel:

Amount	Description	FinalConcentration
1µL	25mMdNTPSolution(N2050L	2.0mM
XµL	1ng-2µgTotalRNA-or-
XµL	5-500ngmRNA(polyAselected)
1µL	Oligo(dT)12-18(500µg/ml)-or-	40µg/mL
1µL	RandomPrimers(125µg/ml)-or	10µg/mL
1µL	GSPPrimer(2pmol)	165µM
XµL	SterileWater	N/A
10µL	TotalVolume

Heatreactionfor5minutesat65°C.Spinbriefly(5sec)topulldowncondensateandplaceimmediatelyonice.
Add1µL10XM-MuLVRTBufferandDNase-freewatertoafinalvolumeof10µLperreaction.
Incubate:
- IfusingOligo(dT)orGSPprimers:2minutes@42°C
- IfusingRandomprimers:2minutes@25°C
Add1µL(200units)thermostableM-MuLVReverseTranscriptaseandmixbygentlypipettingsample.(Note:ifusingrandomprimers,pre-incubatereaction@25°Cfor10minutes).
Incubateat42~72°Cfor45-60minutes.
Inactivateenzymeat95°Cfor10minutes.
Storeproductsat-20°Corproceedtonextstep.

Shipping

4°C

Note

Forresearchuseonly

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
Excellgen	GeneExpresso™Max Transfection Reagent	2	EG-1089	1ml
Excellgen	Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)	4	EG-1069	1 mg
Excellgen	Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg
Excellgen	serine/threonine kinase 33, STK33	7	EG-261	100 µg
Excellgen	Alpha-tubulin Rabbit Polyclonal Antibody	9	EG-1000	100 µg
Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
Excellgen	Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts	13	EG-266	100 µg
Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">