Excellgen/Yeast Poly(A) Polymerase/EG-150/1,000 Units,Excellgen,,Excellgen,+,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：Excellgen/Yeast Poly(A) Polymerase/EG-150/1,000 Units

产品编号：EG-150-1,000Units

市场价：¥9800.00

场地：美国(厂家直采)

产品分类：分子类>核酸酶类>DNA聚合酶>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$490.00

品牌： Excellgen

公司：Excellgen, Inc.

公司分类：

商品介绍

Description	YeastPoly(A)PolymeraseusesATPasasubstratefortemplate-independentadditionofAMPfromATPtothe3´hydroxylterminiofRNAmolecules.Itcanbeusedtoaddpoly(A)tailstoRNAinthefirststepofcloning.ThereactionrequiresMn2+orMg2+,ATPassubstrate,andanyRNAcontaining3'hydroxylterminiasprimer.Substitutionofcordycepin-5'-triphosphate(3'-dATP)forATPresultsinadditionofasingle3'-dAresiduetotheendsoftheRNA,ausefultechniqueforlabelingRNAatthe3'end. Incomparativestudies,YeastPoly(A)PolymeraseworksmoreefficientlythanE.colipoly(A)polymeraseforRNAoligonucleotide-labelingandpoly(A)tailing.Shorterincubationtimesarerequiredfortheyeastenzymeanditisfoundtolabelbothlongandshortsubstratesequallywell.YeastPoly(A)PolymeraseisrecommendedoverT4RNALigasefor3'-endlabelingoflongRNAmolecules.
Applications	Additionofapoly(A)tailtoRNAsynthesizedinvitroforincreasingRNAstABIlityandtranslationefficiencypriortotransferringintoeukaryoticcells CDNAlibraryconstructionusingaprimercontainingpoly(dT)onits3´end. SynthesisofpolyadenylatedRNAfornucleicacidamplification
Source	ArecombinantE.colistraincarryingyeast(Saccharomycescerevisiae)Poly(A)Polymerasegene
UnitDefinition	1unitisdefinedastheamountofenzymethatwillincorporate1nmolofATPintoacid-insolublematerialin10minutesat37°C.
Components	YeastPoly(A)Polymerase:5,000U/mlin20mMTris-HCl,300mMNaCl,1mMDTT,1mMEDTA,50%Glycerol,0.1%Triton®X-100,pH7.5@25°C 5XReactionBuffer:1500mMTris-HCl,pH7.0,3.0mMMnCl2,0.1mMEDTA,1mMDTT,500μg/mlacetylatedBSA,50%glycerol.
QualityControl	Theabsenceofendonuclease,nickingactivity,exonucleaseandRNAseactivityisconfirmedbyappropriatequalitytests.
StorageCondition	-20°C

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号	规格
Excellgen	GeneExpresso™Max Transfection Reagent	2	EG-1089	1ml
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Excellgen	Alpha-tubulin Rabbit Polyclonal Antibody	9	EG-1000	100 µg
Excellgen	Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)	11	EG-156	100 µg
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Excellgen	Flag Tag Mouse Monoclonal Antibody (2C5)	15	EG-277	100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;">