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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/Gst DNA Polymerase, large fragment/EG-200/4,000 Units
产品编号:EG-200-4,000Units
市  场 价:¥1000.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$50.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/Gst DNA Polymerase, large fragment/EG-200/4,000 Units
商品介绍
Description

GstDNAPolymeraseisfunctionallyequivalenttothelargefragmentofBsmPolymeraseIandBstPolymeraseI.RecombinantGstDNAPolymerase(largefragment)has5’->3’polymeraseactivity,butlacks5’->3’exonucleaseand3'→5'exonucleaseactivities.GstDNAPolymerase,LargeFragment,hasastrongstranddisplacementactivityandisactiveinawiderangeoftemperaturesfrom30°Cto63°C,withanoptimumofactivityat60°.ItisanenzymewithhighfunctionalsimilaritytoBstDNAPolymerase,LargeFragment,andcanreplaceitinmostapplicationssuchasrapidamplificationoftraceamountsofDNAtemplatesforsequencingandLAMP.

Applications
  • IsothermalDNAamplificationbythemethodof:
    • Loop-mediatedisothermalamplification(LAMP).
    • Wholegenomeamplification(WGA).
    • Ramificationamplification(RAM).
  • Random-primedDNAlabeling
  • Labelingbyfill-in5'-overhangsofdsDNA
SourceArecombinantE.colistraincarryingthelargefragmentofDNApolymerasegenefromGeobacillusstearoThermophilus
UnitDefinitionOneunitisdefinedastheamountofpolymeraserequiredtocatalyzetheincorporationof10nmolofdNTPintoacid-insolublematerialin30minutesat65°C.
Components
  • GstDNAPolymerase:100,000U/mlin10mMTris-HCl,50mMKCl,1.0mMDithiothreitol,0.1mMEDTA,0.1%TritonX-100,50%Glycerol,pH7.5@25°C
  • 10XReactionBuffer:200mMTris-HCl,100mM(NH4)2SO4,100mMKCl,20mMMgSO4,and1.0%TritonX-100,pH8.8@25°C
QualityControlTheabsenceofendonuclease,nickingactivityandexonucleaseactivityisconfirmedbyappropriatequalitytests.
StorageCondition

-20°C

品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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