| Description | RecombinantTurboHRV3CProteaseencodedbyhumanrhinovirus143Cgeneishighlyspecificfortherecognition"PreScission"sequenceLeu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro(LEVLFQ↓GP)andcleavesbetweentheGlnandGlyresidues.Ithasapolyhistidine(6xHis)tag,whichfacilitateseasyremovaloftheproteaseusingmetalchelateresinsaftercleavagereaction.TurboHRV3CexhibitshighestactivityaroundneutralpHinavarietyofcommonlyusedbuffersattemperaturerangingfrom22to37℃.Italsoretainsrobustactivityat4℃,allowingremovalofpurificationtagsfromtargetproteinsatlowtemperature. |
| Application | Removalofpurificationtagsfromfusionproteins |
| Source | E.coliover-expressingsynthetic(enhanced)formofTEVNIaproteinasegene. |
| Size | 1mg |
| Purification | FPLC |
| Purity | >95%bySDS-PAGE |
| SpecificActivity | >10Units/µg.1UnitofTurboTEVProteasecleaves>85%of3µgofcontrolsubstratein1hourat30oCinRecommendedReactionBuffer:50mMTris-HCl,150mMNaCl,pH8.0 |
| Storage | -20°C. |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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