Description | RecombinantTurboHis-GST-HRV3CProteaseencodedbyhumanrhinovirus143Cgeneishighlyspeckficfor(therecognition"PreScission"sequenceLeu-Glu-Val-Leu-Phe-Gln-↓-Gly-Pro(LEVLFQ↓GP)andcleavesbetweentheGlnandGlyresidues.Itisengineeredasafusionproteinwithbothpolyhistidine(6xHis)andglutathione-S-transferase(GST)tags,whichfacilitateeasyremovaloftheproteaseaftercleavageusingeitherglutathione(GSH)ormetalchelatesresins.TurboHis-GSTHRV3CexhibitshighestactivityaroundneutralpHinavarietyofcommonlyusedbuffersattemperaturerangingfrom22to37℃.Italsoretainsrobustactivityat4℃,allowingremovalofpurificationtagsfromtargetproteinsatlowtemperature |
Applications | Removalofpurificationtagsfromfusionproteins |
Source | E.coliover-expressingsynthetic(enhanced)formofTEVNIaproteinasegene. |
Size | 1mg |
MW | 47.4kD |
SpecificActivity | >1Units/µg.1Uni~sofTuxboHir-GSTHRV3CProteasecleaves>95%of100µgoftargetproteinat4oCfor16hoursinRecommendedReactionBuffer:50mMTris-HCl,150mMNaCl,pH8.0 |
Storage | -20°C. |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
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