| Description | YeastSUMOprotease(alsoknownasUlp),isahighlyactivieandrobustcysteinylproteaseencodedbytheUbl-specificprotease1genefromSaccharomycescerevisiae.ThereareasmanyassixSUMOproteasesinhuman(SENP1–3,SENP5–7).BothhumanandyeastSUMOproteasesrecognizethetertiarystructureoftheubiquitin-like(UBL)protein,i.e.,SUMO-1,SUMO-2andSUMO-3,ratherthanashortstretchofaminoacidsequence,consequently,theseSUMOproteaseswillnotcleavewithinthefusedproteinofinterest.TurboSUMOProteaseisamixtureofcorecatalyticproteasesofbothhumanSENP2andyeastUlp1SUMOproteins,thusSUMO-fusionproteinscanbedigestedbytheTurboSUMOProteaseateither~15°Covernightorat30°Cforafewhours.Afterdigestion,TurboSUMOProteasecanbeeasilyremovedfromthecleavagereactionusingNiagarosebeads |
| Application | RemovalofSUMOtagfromfusionproteins |
| Source | E.coliover-expressingtheC-terminalSENP2andUlp1catalyticproteasedomainsfromhumanandSaccharomycescerevisiae,respectively. |
| Purification | FPLC |
| Purity | >95%bySDS-PAGE |
| Components | 10U/µlTurboSUMOProteasein50mMHepes,pH7.5,150mMNaCl,0.5mMDTT,50%glycerol |
| UnitDefinition | OneunitofSUMOProteaseisdefinedastheamountofenzymeneededtocleave85%of2µgofsubstrateproteinat30°Cor15°Cinonehour |
| RecommendedReactionCondition | SUMOProtease:Add1unitto2µgoftargetSUMOfusionprotein Buffer:50mMTris-HCl,pH8.0,0.1%Igepal(NP-40),150mMNaCl,1mMDTT Temperature:30°C(1~3hours),15°C(overnight) OptimalSaltConcentration:imidazole:<150mM;NaCl:<300mM |
| Storage | -20°C,orat–80°Cforlong-termstorage |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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