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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped FLP recombinase mRNA, NLS-hFLP/EG-1044/10 μg
产品编号:EG-1044
市  场 价:¥7900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$395.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/Pseudouridine Modified 5 Anti-Reverse Cap Analog (ARCA) capped FLP recombinase mRNA, NLS-hFLP/EG-1044/10 μg
商品介绍
Description

FLPrecombinasecatalyzesthesite-specificrecombinationofDNAbetweenFRTsites.Ithasbeenwidelyusedforgeneratingconditionalknockoutmice,mediatedbyFLP/FRTsystem.

CodonoptimizedhFLPisahuman-codonoptimizedThermo-stablemutantformofFLP,withenhancedrecombinaseactivitybya4-10foldhigherthanFLPat37°C.

RecentstudiesdemonstatedthatpseudouridinemodifiedmRNAsignaficantlyreducePKRactivationincells,therebyenhancestranslationoftheencodedproteinafterbeingdeliveredintoculturecellsorinjectedintoanimals.

Pseudouridinemodified5'Anti-ReverseCapAnalog(ARCA)cappedNLS-hFLPrecombinase(humancodon-optimizedNLS-hFLP)mRNAhasbeentestedforFRTrecombinationinFRT-RFP-Stop-FRT-GFPreportercells(e.g.,HEK293,Hela,andNIH3T3),andprimarycells.

Applications

  • exvivoFRTrecombination.
  • invivoFRTrecombination.
Size

5µg

TransfectionProtocols

1.InvitrogenRNAiMAXmRNATransfectionProtocol
2.MirusBioTransIT-mRNAmRNATransfectionProtocol

Storage-20°Cor-80°C
Shippingdryice
References
  1. Kariko,K.etal.IncorporationofpseudouridineintomRNAyieldssuperiornonimmunogenicvectorwithincreasedtranslationalcapacityandBIOLOGicalstABIlity.Mol.Ther.16,1833–1840(2008).
  2. Kariko,K.,Muramatsu,H.,Keller,J.M.&Weissman,D.Increasederythropoiesisinmiceinjectedwithsubmicrogramquantitiesofpseudouridine-containingmRNAencodingerythropoietin.Mol.Ther.20,948–953(2012).
  3. Kormann,M.S.etal.ExpressionoftherapeuticproteinsafterdeliveryofchemicallymodifiedmRNAinmice.Nat.Biotechnol.29,154–157(2011).
  4. Warren,L.etal.HighlyefficientreprogrammingtopluripotencyanddirecteddifferentiationofhumancellswithsyntheticmodifiedmRNA.CellStemCell7,618–630(2010).
品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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