| ProductName | E2F-1(RBAP-1)TranscriptionFactorandRb-mediator |
| Size | 5,000U |
| Description | E2FwasoriginallyidentifiedasatranscriptionfactormediatingthetranscriptionofadenovirusE2gene.Itisaheterodimercomposedoftwostructurallyrelatedsubunits,termedE2FandDP.E2Fisencodedbyatleastfivegenes,E2F-1throughE2F-5,andDPisencodedbyatleasttwogenes,DP-1andDP-2.Allthesegenes(E2F-1throughE2F-5,DP-1andDP-2)havebeenconsideredasmembersoftheE2Fgenefamilybecausetheyhaveanumberofcommonstructuralandfunctionalcharacteristics(1-4).AmongthosemembersoftheE2Ffamily,E2F-1wasthefirstE2Fgeneclonedandcharacterized.TheE2F-1proteinhasbeenshowntointeractwithRBbothinvitroandinvivo(5,6).AlthoughtheE2Fproteinscanfunctionastranscriptionfactorsbythemselves,theyshouldbeconsideredasRB-mediatorsaswellbecausealmosteveryfunctionalresponseofRBprotein(s)requiresthepresenceofE2Fprotein(s). |
| Applications |
|
| Source | Baculovirus–insectcell |
| FusionTag | His |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogramofpurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 1869 |
| Accession# | NM_005225 |
| GeneName | E2Ftranscriptionfactor1 |
| GeneSymbol | E2F1 |
| GeneSynonyms | RBP3;E2F-1;RBAP1;RBBP3 |
| MW | 48.6kDa |
| ProteinSequence | ALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDP DLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKS PGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIA KKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQ RLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEE TVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSR MGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGI RDLFDCDFGDLTPLDF |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Bagchi,S.etal.,(1990)Cell62,659-669. 2.Helin,K.etal.,(1992)Cell70,337-350. 3.Kaelin,WG.etal.,(1992)Cell70,351-364. 4.Shan,B.etal.,(1992)Mol.Cell.Biol.12,5620-5631. 5.Chellappan,SP.etal.,(1991)Cell65,1053-1061. 6.Schwarz,JK.etal.,(1993)EMBOJ.12,1013-1020. |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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