| ProductName | Lelch-likeECH-associatedprotein1,KEAP1 |
| Size | 100µg |
| Description | Keap1hasfourdiscreteproteindomains.TheN-terminalBroadcomplex,TramtrackandBric-à-Brac(BTB)domaincontainstheCys151residue,whichoneoftheimportantcysteinesinstresssensing.Theinterveningregion(IVR)domaincontainstwocriticalcysteineresidues,Cys273andCys288,whichareasecondgroupofcysteinesimportantforstresssensing.Adoubleglycinerepeat(DGR)andC-terminalregion(CTR)domainscollaboratetoformaβ-propellerstructure,whichiswhereKeap1interactswithNrf2. Keap1hasbeenshowntointeractwithNrf2,amasterregulatoroftheantioxidantresponse,whichisimportantfortheameliorationofoxidativestress.Underquiescentconditions,Nrf2isanchoredinthecytoplasmthroughbindingtoKeap1,which,inturn,facilitatestheubiquitinationandsubsequentproteolysisofNrf2.SuchsequestrationandfurtherdegradationofNrf2inthecytoplasmaremechanismsfortherepressiveeffectsofKeap1onNrf2. BecauseNrf2activationleadstoacoordinatedantioxidantandanti-inflammatoryresponse,andKeap1repressesNrf2activation,Keap1hasbecomeaveryattractivedrugtarget.Syntheticoleanetriterpenoidcompounds,knownasantioxidantinflammationmodulators(AIMs)havebeenshowntoblockKeap1-dependentNrf2ubiquitinationandleADIngtothestABIlizationandnucleartranslocationofNrf2andsubsequentinductionofNrf2targetgenes. |
| Source | baculovirus/sf9cells |
| FusionTag | 6XHis |
| PurificationMethod | FPLC |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 9817 |
| Accession# | NM_000546 |
| GeneName | kelch-likeECH-associatedprotein1 |
| GeneSymbol | KEAP1 |
| GeneSynonyms | INrf2;KLHL19 |
| MW | 69.6kDa |
| ProteinSequence | 1MQPDPRPSGAGACCRFLPLQSQCPEGAGDAVMYASTECKAEVTPSQHGNRTFSYTLEDHT |
| StorageBuffer | 20mMTris-Cl(pH8.0),50%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
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Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
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Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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