| ProductName | HighMobilityGroup1protein,HMG1 |
| Size | 10,000U |
| Description | Highmobilitygroup1(HMG1)isa26kDahighlyconservednon-sequence-specificDNA-bindingnuclearprotein(1).MammalianHMG1hastwohomologousDNA-bindingdomains:HMGboxes,AandB(eachof80–90amino-acidresidues),linkedbyashortbasicregiontoanacidicC-terminaldomaincontaining30consecutiveAspandGluresidues(2).HMG1hasbeenimplicatedinanumberoffundamentalBIOLOGicalprocessesincludingtranscription,replicationandrecombination,inwhichitplaysaroleinmanipulatingDNAstructurebybending,looping,compactingorunwinding,orbydirectcontactswithdistinctcellularproteins(3-5).HMG-1canactasarepressor,byinteractingwithTBPtoblockpre-initiationcomplexformation(6,7)orasanactivator,byfacilitatingthebindingofvarioustranscriptionfactorstotheircognateDNAsequences(8,9).Mostrecently,itwasdiscoveredthatHMG-1isalatemediatorofdelayedendotoxinlethalitybyactivatingdownstreamcytokinerelease(10). |
| Applications |
|
| Source | E.coli |
| FusionTag | His |
| PurificationMethod | FPLC |
| UnitDefinition(Activity) | 1unitequalsto1nanogrampurifiedprotein.1unitissufficientforagelmobilityshiftassayina20µlreaction;100unitsaresufficientforprotein-proteininteractionassays. |
| Purity | ~95%asdeterminedbySDS-PAGE |
| GeneID | 3146 |
| Accession# | NM_002128,NP_002119 |
| GeneName | highmobilitygroupbox1 |
| GeneSymbol | HMGB1 |
| GeneSynonyms | HMG1;HMG3;SBP-1 |
| MW | 27.3kDa |
| ProteinSequence | GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKADKARYEREMKTYIPPKGETKKKFKDPNAPKRPPSAFFLFCSEYRPKIKGEHPGLSIGDVAKKLGEMWNNTAADDKQPYEKKAAKLKEKYEKDIAAYRAKGKPDAAKKGVVKAEKSKKKKEEEEDEEDEEDEEEEEDEEDEDEEEDDDDE |
| StorageBuffer | 20mMTris-Cl(pH8.0),20%Glycerol,100mMKCl,1mMDTTand0.2mMEDTA |
| Storage | -20to-80°C. |
| Shipping | 4°Cordryice |
| References | 1.Bianchi,M.E.,etal.,(1989)Science243,1056-1059 2.Bustin,M.,etal.,(1990)Biochim.Biophys.Acta1049,231-243 3.Zappavigna,V.,etal.,(1996)EMBOJ.15,4981-4991 4.Ge,H.,etal.,(1994)J.Biol.Chem.269,17136-17140 5.Zlatanova,Y.etal.,(1998)FASEBJ.12,421-431 6.Stelzer,G.,etal.,(1994)Mol.Cell.Biol.14,4712-4721 7.Lu,W.,etal.,(2000)J.Biol.Chem.Nov10;275(45):35006-12 8.Jayaraman,L.,etal.,(1998)GenesDev.12,462-472 9.Onate,S.,etal.,(1994)Mol.Cell.Biol.14,3376-3391 10.Yang,H.,etal.,(2001)Shock15,247-253 |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |
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