Description | GeneExpresso™PlusDNAInVitroTranfectionReagentisenhancedversionofGeneExpresso™DNAInVitroTransfectionReagent.GeneExpresso™Plusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonsomemammaliancellsincomparisonofitspreviousversion.GeneExpresso™PluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GeneExpresso™Plusreagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates. |
Advantage |
GeneExpresso™Plusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGeneExpresso™PlusandL2Krespectivelypermanufacturer'sprotocols.Theefficiencywaschecked48hoursposttransfection. LeftPanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells. Rightpanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithtranfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry. Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGeneExpresso™PlusandLipofectamineLTXwerequantifiedtobe2.1x106and9.3x106tu/mlrespectively. |
Protocol | DNATransfectionProtocol |
StorageConditions | Uponarrivalstorethisproductat-20oCor4oC.Ifstoredproperly,theproductwillbestablefor12monthsorlonger.Productshippedatambienttemperature. |
Discount | NewCustomer:Pleaseenterdiscountcode98Onlytoreceiveupto$100discount(1ml). ReturnCustomer:Volumediscountorquotationisavailable(sendemailtocrm@Excellgen.com) 100µltrialsampleisfornewcustomersonly |
FreeSample | RequestaFreeSample |
德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。
No.
货号 | 规格 | |||
Excellgen | GeneExpresso™Max Transfection Reagent | 2 | EG-1089 | 1ml |
Excellgen | Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre) | 4 | EG-1069 | 1 mg |
Excellgen | Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 1 mg | |||
Excellgen | serine/threonine kinase 33, STK33 | 7 | EG-261 | 100 µg |
Excellgen | Alpha-tubulin Rabbit Polyclonal Antibody | 9 | EG-1000 | 100 µg |
Excellgen | Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b) | 11 | EG-156 | 100 µg |
Excellgen | Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts | 13 | EG-266 | 100 µg |
Excellgen | Flag Tag Mouse Monoclonal Antibody (2C5) | 15 | EG-277 | 100 µg <span line-height:2;"="" style="outline: none; font-size: 14px; font-family: "Microsoft YaHei"; line-height: 2;"> |