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主营类目:Ferrous Scraps,Petroleum By-Products,蛋白肽,逆转录酶等
℡ 4000-520-616
℡ 4000-520-616
Excellgen/GeneExpresso™ Plus DNA In Vitro Tranfection Reagent/EG-1032/
产品编号:EG-1032
市  场 价:¥4760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$238.00
品      牌: Excellgen
公      司:Excellgen, Inc.
公司分类:
Excellgen/GeneExpresso™ Plus DNA In Vitro Tranfection Reagent/EG-1032/
商品介绍
DescriptionGeneExpresso™PlusDNAInVitroTranfectionReagentisenhancedversionofGeneExpresso™DNAInVitroTransfectionReagent.GeneExpresso™Plusisformulatedbyadditionofaboosterpeptide,givingrisetoupto20timeshigherefficiencyonsomemammaliancellsincomparisonofitspreviousversion.GeneExpresso™PluswasshowntoefficientlydelivergenestovariousestablishedcelllinesaswellasprimarycellsincludingHEK293,293T,293E,CHO,COS1,HeLa,NIH3T3,insectcelllines(Sf9andSf21)andavarietyofothereucaryoticcelllineswithlesstoxicity.GeneExpresso™Plusreagent,1.0ml,issufficientfor300to600transfectionsin24wellplatesor150to300transfectionsin6wellplates.
Advantage
  • Cell-dependent3~20timeshigherefficiency
  • Topchoiceforhard-to-transfectcells
  • EquallygoodforverylongDNAs(upto2.0mb)
  • Efficiencyboostedinthepresenceofserumandantibioticsformostofcelltypes
  • Exceptionalhighlevelsofrecombinantproteinproduction
ComparisonsofTransfectionEfficiencyofGeneExpresso™PlusInVitroDNATransfectionReagentwiththeLeADIngBrandNameProducts

GeneExpresso™Plusistopchoiceforhard-to-transfectcells.EfficiencycomparisonofGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)withLipofectamine2000(L2K,rightpanel)onprimaryculturedrataorticsmoothmusclecells.AnucleartargetedGFPDNAwastransfectedwithGeneExpresso™PlusandL2Krespectivelypermanufacturer'sprotocols.Theefficiencywaschecked48hoursposttransfection.

LeftPanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithleadingproductsincludingLipofectamine2000(L2K)andLipofectamineLTX(LTX)onlentivirusproductionon293Tcells.
Rightpanel:ComparisonofGeneExpresso™PlusInVitroDNATransfectionReagentwithtranfectionreagentsfromothervendorsinludingLipofectamine2000(L2K)andFuGene6onHelacells.HeLacellsweretransfectedwithplasmidDNAencodingGFP.Transfectionswereperformedaccordingtomanufacturersrecommendationsandanalysedbyflowcytometry.


Exceptionalhightitersoflentivirusgeneratedfrom293Tcells.ThreeDNAsco-transfectedwithGeneExpresso™PlusInVitroDNATransfectionReagent(leftpanel)incomparisonofLipofectamineLTX(rightpanel).1.0and10microlitersvirussupernatantfrom293Tcellswereusedtocounter-infected293FcellswhichwasthenpassedFACS.Thenumbersattheupperrightcornerindicatethepercentageoftransducedcells.ThetitersoflentivirusgeneratedwithGeneExpresso™PlusandLipofectamineLTXwerequantifiedtobe2.1x106and9.3x106tu/mlrespectively.
ProtocolDNATransfectionProtocol
StorageConditionsUponarrivalstorethisproductat-20oCor4oC.Ifstoredproperly,theproductwillbestablefor12monthsorlonger.Productshippedatambienttemperature.
Discount

NewCustomer:Pleaseenterdiscountcode98Onlytoreceiveupto$100discount(1ml).

ReturnCustomer:Volumediscountorquotationisavailable(sendemailtocrm@Excellgen.com)

100µltrialsampleisfornewcustomersonly

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品牌介绍

德国Excellgen特色产品包括Cre重组蛋白、ExcellScript 耐热 M-MuLV (MMLV) 逆转录酶、FLP重组蛋白、GeneExpresso™ Max转染试剂。

No.

货号

规格




Excellgen

GeneExpresso™Max Transfection Reagent

2

EG-1089

1ml

Excellgen

Cre Recombinase, TAT-Cre (Tat-NLS-Cre, HTNC, HTNCre)

4

EG-1069

1 mg

Excellgen

Human Protein Kinase Alpha, PKA, C alpha, cAMP-dependent protein 

1 mg




Excellgen

serine/threonine kinase 33, STK33

7

EG-261

100 µg

Excellgen

Alpha-tubulin Rabbit Polyclonal Antibody

9

EG-1000

100 µg

Excellgen

Recombinant Human Interferon alpha-2b, IFN alpha 2b, (rHuIFN-α2b)

11

EG-156

100 µg

Excellgen

Thermus aquaticus Muts DNA mismatch repair protein, 6X His-Taq-Muts

13

EG-266

100 µg

Excellgen

Flag Tag Mouse Monoclonal Antibody (2C5)

15

EG-277 

100 µg

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