Description

E. coli Uracil-DNA Glycosylase (UDG), also known as Uracil N-Glycosylase (UNG), catalyses the release of uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from short oligonucleotides (<6 bases), or RNA substrates.

Applications
  • Digestion of uracil-containing DNA
SourceAn E. coli strain that carries the ung (Uracil-DNA Glycosylase) gene from E. coli K-12.
Unit DefinitionOne unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA.
Components
  • E coli UDG: 5,000 units/ml in 10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4 @ 25°C
  • 10X Reaction Buffer: 200mM Tris-HCI, 10 mM DTT, 10 mM EDTA, pH 8.0 @ 25°C
Quality Control

The absence of single and double-strand exonuclease, and endodeoxyribonucleases is confirmed by appropriate quality tests

Storage Condition

-20 °C